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Transcardiac Perfusion of the Mouse for Brain Tissue Dissection and Fixation
Jinyun Wu1, Yuqi Cai1, Xiaoyang Wu1
1Institutes of Brain Sciences, Fudan University, Shanghai 200032, China.
Bio-Protocol
|April 2, 2021
Abstract:
Transcardiac perfusion with saline followed by 4% paraformaldehyde (PFA) is widely used to clear blood and preserve brain for immunostaining or in situ hybridization. PFA breaks into formaldehyde in solution, which cross-link protein and DNA molecules to preserve tissue and cell structure. Here we provide a step by step guide for performing this procedure in mouse.
