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Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen
Published on: July 15, 2018
The complete mitochondrial genome of spiny spooner Etisus dentatus (Herbst, 1785) using high-throughput sequencing
Hongtao Liu1,2, MingHui Shen2
1Ministry of Education, Key Laboratory of Utilization and Conservation for Tropical Marine Bioresources (Hainan Tropical Ocean University), Sanya, China.
Abstract:
The whole mitochondrial genome of the spiny spooner Etisus dentatus collected from the South China Sea was determined for the first time using high-throughput sequencing. The circular mitogenome of E. dentatus is 15,884 bp, with 13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes. The base composition is significantly biased (A, G, T, and C was 37.9%, 17.8%, 34.0%, and 10.3%, respectively) with A + T contents of 71.9%. Among 13 PCGs, all PCGs use a normal ATN as the start codon, and ten of them end with TAA or TAG except COX1, COX2 and CYTB gene using a single T as the stop codon. The phylogenetic tree showed that E. dentatus forms a cluster E. anaglyptus while Atergatis integerrimus form a sister group to this cluster.
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