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Updated: Nov 10, 2025
Author Spotlight: Studying hiPSC-Derived Endothelial Cells Cultured Under Fluidic-Mediated Mechanical Stimulation
Published on: July 28, 2023
Differentiation and characterization of human iPSC-derived vascular endothelial cells under physiological shear
Nadia O Abutaleb1, George A Truskey1
1Department of Biomedical Engineering, Duke University, Durham, NC 27713, USA.
Abstract:
Induced pluripotent stem cells (iPSCs) offer a potentially unlimited source to generate endothelial cells (ECs) for numerous applications. Here, we describe a 7-day protocol to differentiate up to 55 million vascular endothelial cells (viECs) from 3.5 million human iPSCs using small molecules to regulate specific transcription factors. We also describe a parallel-plate flow chamber system to study EC behavior under physiological shear stress. For complete details on the use and execution of this protocol, please refer to Atchison et al. (2020).
