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Green tea polyphenols inhibit malignant melanoma progression via regulating circ_MITF/miR-30e-3p/HDAC2 axis

Kejia Wu1, Yuegang Wei2, Ye Yu1

  • 1Department of Dermatology, Changzhou Hospital of Traditional Chinese Medicine, Changzhou, China.

Biotechnology and Applied Biochemistry
|April 2, 2021
PubMed

Insights

Green tea polyphenols (GTPs) show anticancer effects against malignant melanoma by inhibiting cell growth and promoting apoptosis. GTPs regulate the circ_MITF/miR-30e-3p/HDAC2 pathway, offering a potential therapeutic strategy for melanoma.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Malignant melanoma is a significant health concern.
  • Green tea polyphenols (GTPs) exhibit anticancer properties, but their mechanisms in melanoma are unclear.
  • Understanding GTPs' molecular targets is crucial for therapeutic development.

Purpose of the Study:

  • To elucidate the mechanisms underlying the anticarcinogenic effects of GTPs in malignant melanoma.
  • To investigate the role of circ_MITF, miR-30e-3p, and HDAC2 in GTPs' action.
  • To evaluate the therapeutic potential of GTPs in preclinical melanoma models.

Main Methods:

  • In vitro cellular function assays (proliferation, migration, invasion, apoptosis, EMT).
  • Analysis of circ_MITF, miR-30e-3p, and HDAC2 expression and interactions.
  • In vivo xenograft studies in nude mice.

Main Results:

  • GTPs inhibited melanoma cell proliferation, migration, invasion, EMT, and promoted apoptosis.
  • GTPs decreased elevated circ_MITF expression, which was linked to melanoma progression.
  • GTPs function via the circ_MITF/miR-30e-3p/HDAC2 axis, suppressing tumor growth in vivo.

Conclusions:

  • GTPs suppress melanoma progression through the circ_MITF/miR-30e-3p/HDAC2 pathway.
  • This pathway represents a potential therapeutic target for malignant melanoma intervention.
  • GTPs demonstrate promise as a natural therapeutic agent for melanoma.
Keywords:
GTPsHDAC2circ_MITFmalignant melanomamiR-30e-3p

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