Biodegradation of diisononyl phthalate by a consortium of saline soil bacteria: optimisation and kinetic...

Diisononyl phthalate (DINP) is one of plasticisers most employed in the production of plastic materials and belongs to the most important environmental contaminants. In this work, a consortium of saline soil bacterial (SSB) capable of degrading DINP is presented. The genera of SSB-consortium were Serratia sp., Methylobacillus sp., Achromobacter sp., Pseudomonas sp., Stenotrophomonas sp., Methyloversatilis sp., Delftia sp. and Brevundimonas sp. Response surface methodology (RSM) study was employed to optimise and evaluate the culture conditions to improve the biodegradation of DINP. The optimal conditions were a pH 7.0, 31 °C and an initial DINP concentration of 500 mg L^-1, resulting in almost complete biodegradation (99%) in 168 h. DINP degradation followed a first-order kinetic model, and the half-life was 12.76 h. During the biodegradation of DINP, 4-derived compounds were identified: monoisononyl phthalate, methyl nonyl phthalate, iso-nonanol and dimethyl phthalate. The metabolite profiling indicated that DINP was degraded through simultaneous pathways of de-esterification and β-oxidation. Results suggest that the SSB-consortium could be useful for efficient biodegradation of the DINP-contaminated environments. KEY POINTS: • DINP degradation is mediated by de-esterification and β-oxidation processes. • Temperature and the concentration of the substrate are key factors for DINP biodegradation • The SSB-consortium has the ability to biodegrade 99% of DINP (500 mg L^-1).