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Updated: Nov 10, 2025

Fluorescence In Situ Hybridization on DNA Halo Preparations to Reveal Whole Chromosomes, Telomeres and Gene Loci
Published on: March 4, 2021
Sizing chromosomes and megaplasmids in haloarchaea
P López-García1, R Amils1, J Antón1
1Centro de Biologí Molecular, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain.
Abstract:
PFGE was used for the genomic analysis of different species and strains belonging to four out of the six recognized haloarchaeal (halobacterial) genera. All of them were found to carry one chromosome from 1.8-3 Mb, and usually several, but at least one, large plasmids of approximately 90-680 kb, which were detected in supercoiled and linear forms. From the data gathered, chromosomal size appears to be conserved at genus level, whereas plasmid composition and size seems to be subjected to certain variability.
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