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Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins
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Molecular Characterization of Ahp2, a Lytic Bacteriophage of Aeromonas hydrophila

Jian-Bin Wang1, Mei-Shiuan Yu2,3, Tsai-Tien Tseng4

  • 1Laboratory of Microbial Genetics, Institute of Medical Sciences, Tzu Chi University, No. 701, Sec. 3, Zhongyang Rd., Hualien 97004, Taiwan.

Viruses
|April 3, 2021
PubMed

Insights

A novel myophage, vB_AhyM_Ahp2 (Ahp2), was isolated and characterized for its ability to infect Aeromonas hydrophila. This lytic phage shows rapid adsorption and a high burst size, making it a potential candidate for phage therapy against A. hydrophila infections.

Area of Science:

  • Microbiology
  • Virology
  • Bacteriology

Background:

  • Aeromonas hydrophila is an opportunistic pathogen affecting various hosts, including humans.
  • There is a need for effective treatments against A. hydrophila infections.

Purpose of the Study:

  • To isolate and characterize a novel myophage, vB_AhyM_Ahp2 (Ahp2), with lytic activity against Aeromonas hydrophila.
  • To evaluate the potential of Ahp2 as a therapeutic agent.

Main Methods:

  • Isolation and characterization of the myophage Ahp2.
  • Adsorption, latent period, and burst size assays.
  • Genome sequencing and analysis of Ahp2.
  • Host lysis assays and investigation of lysogenization.

Main Results:

  • Ahp2 efficiently adsorbed to A. hydrophila (96% in 18 min) with a latent period of 15 min and burst size of 142 PFU/cell.
  • Ahp2 possesses a linear double-stranded DNA genome (47,331 bp) with 57% GC content and shares genomic organization with other Aeromonas phages.
  • Ahp2 completely lysed A. hydrophila AH300206 within 3.5 h at a MOI of 0.0001 without lysogenization.

Conclusions:

  • Ahp2 is a potent lytic phage against Aeromonas hydrophila.
  • The characterized properties of Ahp2 suggest its potential as a therapeutic agent in phage cocktails for treating A. hydrophila infections.
Keywords:
Aeromonas hydrophilaAeromonas phageAhp2myophage

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