Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A model of hypoxic renal failure.

J A Galat1, A V Robinson, R S Rhodes

  • 1Department of Surgery, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106.

The Journal of Surgical Research
|June 1, 1988
PubMed
Summary

This study shows that short hypoxic insults cause temporary kidney dysfunction, while longer ones lead to irreversible damage. This research models hypoxic acute renal failure (ARF) to better understand kidney injury.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The carbon footprint of group and save in elective and emergency surgery.

Annals of the Royal College of Surgeons of England·2025
Same author

The management of chyle leak post-oesophagectomy for oesophageal carcinoma: a systematic review.

Annals of the Royal College of Surgeons of England·2021
Same author

Managing infected seroma post-lymphadenectomy; A retrospective cohort study and cost analysis in melanoma patients.

Journal of plastic, reconstructive & aesthetic surgery : JPRAS·2021
Same author

New technology and new approaches to surgical education.

Bulletin of the American College of Surgeons·2007
Same author

Topical phenytoin treatment of stage II decubitus ulcers in the elderly.

The Annals of pharmacotherapy·2001
Same author

Basic science curriculum in vascular surgery residency.

Journal of vascular surgery·2001

Area of Science:

  • Nephrology
  • Physiology
  • Pathology

Background:

  • Ischemic acute renal failure (ARF) involves hypoxia, nutrient deficiency, and waste buildup.
  • Developing accurate models for ARF is crucial for understanding kidney injury.

Purpose of the Study:

  • To characterize the renal response to graded hypoxic insults in an isolated perfused rat kidney model.
  • To investigate the hypoxic component of postischemic renal dysfunction.

Main Methods:

  • Isolated perfused rat kidneys were subjected to 10 or 30 minutes of hypoxia.
  • Perfusate oxygen tension was reduced from ~550 to 50 mm Hg.
  • Renal function parameters including GFR, urine flow, sodium reabsorption, and oxygen consumption were measured.

Main Results:

  • 10 minutes of hypoxia caused transient tubular dysfunction and a 40% decrease in GFR.
  • 30 minutes of hypoxia resulted in irreversible GFR reduction (85%), decreased urine flow, and severe tubular dysfunction.
  • Hypoxia significantly reduced kidney oxygen consumption.

Conclusions:

  • Renal dysfunction severity correlates with hypoxic insult duration, mirroring clinical ARF spectrum.
  • This isolated perfused kidney model effectively simulates hypoxic ARF, enabling targeted research into hypoxia's role in kidney injury.

Related Experiment Videos