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Density gradients for isolation of mononuclear blood cells for magnesium analysis by electron probe X-ray
G R Hook1, J M Hosseini, R J Elin
1Surgical Neurology Branch, National Institutes of Neurological and Communicative Disorders and Stroke, Bethesda, MD.
Abstract:
We present a mononuclear blood cell (MBC) isolation method for magnesium analysis by electron probe X-ray microanalysis. We compare the inorganic elemental composition of individual MBC isolated by either arabinogalactan (Stractan) or conventional Ficoll-Hypaque density gradients. We find that the MBC isolated with Stractan have the expected cellular element composition, but MBC isolated with Ficoll-Hypaque are contaminated with iodine and sodium. We discuss the source and significance of iodine and sodium contamination of Ficoll-Hypaque isolated MBC and recommend the Stractan separation method for electron probe X-ray microanalysis.