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Growth of Cartilage and Bone Tissue01:27

Growth of Cartilage and Bone Tissue

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Chondrocytes form a temporary cartilaginous model by dividing and secreting a thick gel-like extracellular matrix. Once the chondrocytes undergo programmed cell death, osteoblasts enter the site of the cartilaginous model. The process of replacing the temporary cartilaginous model with bone in an ordered manner is called endochondral ossification. In endochondral ossification, not all of the cartilage is replaced by bone tissue. Some cartilage that performs a protective and supportive function...
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Development of Injectable Polydactyly-Derived Chondrocyte Sheets.

Shiho Wasai1,2, Eriko Toyoda1,2, Takumi Takahashi1,2

  • 1Department of Orthopaedic Surgery, Surgical Science, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa 259-1193, Japan.

International Journal of Molecular Sciences
|April 3, 2021
PubMed
Summary

Injectable polydactyly-derived chondrocyte sheet mini-fragments (PD sheets-mini) show promise for less invasive cartilage repair in osteoarthritis. These fragments maintain key characteristics and viability after injection, offering a potential alternative to traditional surgical transplantation.

Keywords:
cartilage regenerationcell sheetminimally invasive treatmentosteoarthritis

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Area of Science:

  • Regenerative Medicine
  • Orthopedics
  • Biomaterials Science

Background:

  • Osteoarthritis causes articular cartilage damage, often treated with invasive procedures.
  • Current treatments using polydactyly-derived chondrocyte sheets (PD sheets) require highly invasive surgery.
  • Developing less invasive methods for cartilage repair is crucial for patient recovery and treatment accessibility.

Purpose of the Study:

  • To develop and evaluate injectable fragments of PD sheets (PD sheets-mini) as a less invasive alternative for cartilage repair.
  • To compare the characteristics and efficacy of PD sheets-mini with conventional PD sheets.
  • To assess the impact of needle gauge and injection on the viability and function of PD sheets-mini.

Main Methods:

  • Culturing polydactyly-derived chondrocytes in RepCell™ or conventional inserts.
  • Characterizing PD sheets-mini and PD sheets using cell counts, viability assays, histology, ELISA, qPCR, and flow cytometry.
  • Evaluating PD sheets-mini viability under different injection conditions (needle gauge, syringe only).

Main Results:

  • PD sheets-mini produced comparable amounts of humoral factors to PD sheets.
  • Histological analysis revealed no significant differences between PD sheets and PD sheets-mini.
  • Cartilage-related gene expression, except for COL2A1, was similar between both types of sheets.
  • Cell viability of PD sheets-mini was not significantly affected by needle gauge size or injection process.

Conclusions:

  • PD sheets-mini possess characteristics similar to PD sheets.
  • Injectable PD sheets-mini demonstrate feasibility for intra-articular transplantation via less invasive methods.
  • This approach holds potential for a minimally invasive treatment for articular cartilage damage in osteoarthritis.