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Updated: Nov 10, 2025

Laser-Induced Fluorescence Emission L.I.F.E. as Novel Non-Invasive Tool for In-Situ Measurements of Biomarkers in Cryospheric Habitats
Published on: October 26, 2019
A Compact LIF Spectrometer for in-Field Operation in Polar Environments
Lorenzo Palombi1, Valentina Raimondi1
1"Nello Carrara" Institute of Applied Physics-National Research Council, I-50019 Sesto Fiorentino, FI, Italy.
A new compact laser-induced fluorescence (LIF) spectrometer, SFIDA-405, was developed for polar field studies. This portable instrument successfully analyzed biological soil crusts in the Arctic and Antarctic.
Area of Science:
- Environmental science
- Spectroscopy
- Polar research
Background:
- In-situ analysis of biological soil crusts in polar regions is challenging.
- Existing spectroscopic instruments may not be suitable for harsh, remote environments.
Purpose of the Study:
- To develop and test a compact, portable laser-induced fluorescence (LIF) spectrometer for in-field operation in polar environments.
- To assess the instrument's capability for studying biological soil crusts.
Main Methods:
- A novel compact LIF spectrometer (SFIDA-405) utilizing 405 nm excitation was designed.
- The instrument features an optical-fiber coupled measurement head and operates on battery power (<1.6 kg).
- Field campaigns in the Arctic and Antarctic were conducted for instrument validation.
Main Results:
- The SFIDA-405 prototype demonstrated successful in-field operation in polar conditions.
- It achieved a limit of detection for chlorophyll better than 5 ng/cm2.
- The first in-situ LIF spectra of Antarctic biological soil crusts were acquired.
Conclusions:
- The SFIDA-405 is a viable, portable tool for in-situ spectroscopic analysis in extreme polar environments.
- The instrument shows potential for diverse applications beyond biological soil crust studies.
- This work represents a significant advancement in polar field spectroscopy.
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