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Carbon Dot/Naphthalimide Based Ratiometric Fluorescence Biosensor for Hyaluronidase Detection
Pushap Raj1, Seon-Yeong Lee2, Tae Yoon Lee1,2
1Department of Convergence System Engineering and Department of Biomedical Engineering, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Korea.
Materials (Basel, Switzerland)
|April 3, 2021
Summary
A new fluorescence assay detects hyaluronidase (HAase), a bladder cancer biomarker. This sensitive method aids early disease diagnosis by measuring HAase levels in physiological conditions.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Oncology
Background:
- Bladder cancer is a significant cause of mortality in genitourinary cancers.
- Elevated hyaluronidase (HAase) levels are linked to bladder cancer, serving as a potential early diagnostic biomarker.
- A simple and sensitive enzymatic assay is needed for early HAase detection.
Purpose of the Study:
- To develop a simple, sensitive, and ratiometric fluorescence assay for detecting hyaluronidase (HAase).
- To establish a method for early diagnosis of bladder cancer using HAase as a biomarker.
Main Methods:
- Constructed a fluorescence assay using cationic carbon dots and naphthalimide adsorbed onto hyaluronic acid.
- Utilized a Förster resonance energy transfer (FRET) mechanism between carbon dots and naphthalimide.
- HAase enzyme activity was measured by monitoring FRET changes induced by hyaluronic acid cleavage.
Main Results:
- Achieved a sensitive ratiometric fluorescence assay for HAase detection.
- Established a detection limit of 0.09 U/mL, below HAase levels in normal human fluids.
- Demonstrated the assay's potential for diagnosing HAase-related diseases.
Conclusions:
- The developed assay is simple, sensitive, and suitable for detecting HAase under physiological conditions.
- This assay shows promise for the early diagnosis of bladder cancer and other HAase-related conditions.
