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Operating and Biocontainment Procedures of a Facility for Laboratory Mice with a Natural Microbiome: Immunophenotyping Procedure
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Processing Matters in Nutrient-Matched Laboratory Diets for Mice-Microbiome.

Jasmin Wenderlein1, Linda F Böswald2, Sebastian Ulrich1

  • 1Chair of Bacteriology and Mycology, Institute for Infectious Diseases and Zoonosis, Department of Veterinary Sciences, Faculty of Veterinary Medicine, LMU Munich, Veterinärstr. 13, 80539 Munich, Germany.

Animals : an Open Access Journal From MDPI
|April 3, 2021
PubMed
Summary
This summary is machine-generated.

Diet processing affects the gut microbiome in mice. Different feed forms significantly altered the abundance of key bacterial species, impacting research findings.

Keywords:
dietfeed processingintestinal microbiomelaboratory mousestarch gelatinization

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Area of Science:

  • Microbiology
  • Animal Nutrition
  • Gastroenterology

Background:

  • Host diet composition is a primary determinant of gut microbiome structure.
  • Commercial laboratory mouse diets, despite identical labels, can vary in nutrient composition and starch gelatinization due to different production methods (e.g., extrusion vs. pelleting).
  • These variations may influence nutrient digestibility and subsequent host physiological responses.

Purpose of the Study:

  • To investigate the impact of different feed processing methods (extruded vs. pelleted diets) on the gut microbiome composition in C57BL/J6 mice.
  • To determine if variations in diet processing influence the relative abundances of specific microbial taxa across different gastrointestinal regions.

Main Methods:

  • Forty-eight C57BL/J6 mice were divided into two groups and fed either extruded or pelleted diets for eight weeks, with two biological replicates.
  • Samples were collected from five gastrointestinal regions (stomach, small intestine, cecum, large intestine) and feces.
  • Microbiome analysis was performed using 16S rRNA gene amplicon sequencing.

Main Results:

  • Significant differences in the relative abundances of Muribaculaceae species were observed between experimental replicates.
  • Mice fed pelleted diets exhibited higher relative abundances of Lactobacillus species in their gastrointestinal contents compared to mice fed extruded diets.
  • These findings suggest that feed processing influences microbial composition.

Conclusions:

  • Dietary processing methods, specifically extrusion versus pelleting, significantly impact the gut microbiome composition in laboratory mice.
  • Variations in starch gelatinization and ingredient composition resulting from different feed processing methods are key drivers of these microbial shifts.
  • These findings highlight the potential for feed processing to introduce bias in microbiome research, affecting animal experiments and data interpretation.