Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Advancing the predictive techno-economic and lifecycle assessment of prairie grass and manure co-digestion for renewable natural gas applications.

Frontiers in bioengineering and biotechnology·2025
Same author

Assessment of the Sperm DNA Oxidative Damage by Immunofluorescence and Flow Cytometry.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Analysis of the Chromatin Structure by Chromomycin A3 (CMA3) and Flow Cytometry.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Effect of Semen Collection in the Metabolite and Hormonal Content of Rabbit Seminal Plasma.

Veterinary medicine and science·2025
Same author

Seasonality analysis on cryopreserved doses from the autochthonous cattle breeds Asturiana de la Montaña and Asturiana de los Valles.

Reproduction in domestic animals = Zuchthygiene·2024
Same author

Application of Flow Cytometry Using Advanced Chromatin Analyses for Assessing Changes in Sperm Structure and DNA Integrity in a Porcine Model.

International journal of molecular sciences·2024

Related Experiment Video

Updated: Nov 10, 2025

Cryopreservation of Zebrafish Spermatogonia by Whole Testes Needle Immersed Ultra-Rapid Cooling
13:32

Cryopreservation of Zebrafish Spermatogonia by Whole Testes Needle Immersed Ultra-Rapid Cooling

Published on: March 4, 2018

8.6K

Cold-Shock Test Is a Practical Method for Selecting Boar Ejaculates Yielding Appropriate Seminal Plasma for

Estíbaliz Lacalle1, Andrea Núñez1, Estela Fernández-Alegre2

  • 1Institute of Animal Health and Cattle Development (INDEGSAL), Universidad de León, 24071 León, Spain.

Animals : an Open Access Journal From MDPI
|April 3, 2021
PubMed
Summary

Adding seminal plasma (SP) to cryopreserved boar semen improves post-thaw quality. A cold-shock test (CST) can identify resistant SP (SPr) that offers additional benefits for sperm motility and integrity in artificial insemination (AI).

Keywords:
DNA fragmentationboarchromatin statuscold-shock testcryopreserved semendata clusteringosmotic resistance testseminal plasmasperm motilitysperm physiology

More Related Videos

A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization
11:01

A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization

Published on: July 6, 2009

31.6K
Handling and Treatment of Male European Eels Anguilla anguilla for Hormonal Maturation and Sperm Cryopreservation
08:24

Handling and Treatment of Male European Eels Anguilla anguilla for Hormonal Maturation and Sperm Cryopreservation

Published on: January 28, 2018

8.0K

Related Experiment Videos

Last Updated: Nov 10, 2025

Cryopreservation of Zebrafish Spermatogonia by Whole Testes Needle Immersed Ultra-Rapid Cooling
13:32

Cryopreservation of Zebrafish Spermatogonia by Whole Testes Needle Immersed Ultra-Rapid Cooling

Published on: March 4, 2018

8.6K
A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization
11:01

A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization

Published on: July 6, 2009

31.6K
Handling and Treatment of Male European Eels Anguilla anguilla for Hormonal Maturation and Sperm Cryopreservation
08:24

Handling and Treatment of Male European Eels Anguilla anguilla for Hormonal Maturation and Sperm Cryopreservation

Published on: January 28, 2018

8.0K

Area of Science:

  • Animal Science
  • Reproductive Biology
  • Biotechnology

Background:

  • Artificial insemination (AI) in the swine industry faces challenges with cryopreserved semen quality.
  • Seminal plasma (SP) addition shows potential to enhance post-thaw sperm parameters, but its effectiveness can vary.
  • A simple cold-shock test (CST) may differentiate SP suitability for cryopreservation.

Purpose of the Study:

  • To evaluate the impact of seminal plasma (SP) from cold-shock test (CST)-classified ejaculates on post-thaw boar sperm quality.
  • To determine if CST-resistant SP (SPr) offers superior benefits compared to CST-sensitive SP (SPs) or no SP addition.

Main Methods:

  • A cold-shock test (CST) classified 63 boar ejaculates into resistant (SPr) and sensitive (SPs) groups.
  • Frozen-thawed spermatozoa were incubated with MR-A® extender, 20% SPr, or 20% SPs for 4 hours.
  • Sperm motility, kinematics, viability, apoptosis, membrane integrity, acrosomal damage, chromatin structure, and disulfide levels were analyzed.

Main Results:

  • Seminal plasma (SP) addition significantly improved total and progressive motility, with a greater effect observed with SPr.
  • SP inclusion decreased kinematic parameters (VCL, VAP, ALH, BCF) but did not affect sperm viability.
  • SP increased apoptotic and membrane disorder ratios, and acrosomal damage, while elevating free thiols, indicating disulfide bridge reduction.

Conclusions:

  • Seminal plasma (SP) exerts a stimulatory effect on post-thaw boar sperm, with CST-resistant SP (SPr) providing additional benefits.
  • The CST is a practical method for selecting ejaculates with superior SP for improving cryopreserved semen quality in AI.
  • Further in vivo studies are warranted to confirm the benefits of SPr in artificial insemination programs.