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Using Primary Neurosphere Cultures to Study Primary Cilia

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Published on: April 14, 2017

Investigating Primary Cilia during Peripheral Nervous System Formation.

Elkhan Yusifov¹, Alexandre Dumoulin¹, Esther T Stoeckli¹

¹Department of Molecular Life Sciences and Neuroscience Center Zurich, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland.

International Journal of Molecular Sciences

|April 3, 2021

Summary

Primary cilia are essential for vertebrate embryonic development and nervous system formation. This study found primary cilia in neural crest cells and their derivatives, suggesting a role in peripheral nervous system development.

Keywords:

DRG PNS boundary cap cells ciliogenesis neural circuit formation neural crest cells primary cilium sympathetic ganglia

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Last Updated: Nov 10, 2025

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Published on: April 14, 2017

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Volumetric Imaging and Analysis of Primary Cilia in Musculoskeletal Tissue using the ARL13B-CENTRIN-2 Mouse Model

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Published on: March 28, 2025

Area of Science:

Developmental Biology
Neuroscience
Cell Biology

Background:

The primary cilium is a crucial organelle in vertebrate embryonic development, acting as a signaling hub for pathways like Sonic Hedgehog.
Dysregulation of ciliogenesis or ciliary function causes human ciliopathies, severe developmental disorders.
While the primary cilium's role in central nervous system development is recognized, its function in peripheral nervous system formation remains largely unexplored.

Purpose of the Study:

To investigate the presence and potential role of primary cilia in neural crest cells and their derivatives during peripheral nervous system development in vivo.
To compare ciliation patterns in different neuronal populations from the peripheral and central nervous systems.

Main Methods:

In vivo studies using developing chicken embryos.
Microscopic examination of neural crest cells, sensory neurons, and boundary cap cells.
Analysis of neuronal cultures from peripheral and central nervous system populations.

Main Results:

Primary cilia were detected in neural crest cells, sensory neurons, and boundary cap cells during critical stages of peripheral nervous system formation.
Distinct differences in ciliation were observed between neuronal cultures from peripheral and central nervous system origins.

Conclusions:

Neural crest cells and their derivatives possess primary cilia during peripheral nervous system development.
These findings establish a basis for future research into the specific functions of primary cilia in peripheral nervous system formation, both in vivo and in vitro.