Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Special Staining Techniques01:13

Special Staining Techniques

773
Specialized staining techniques play a vital role in microbiology by enabling the visualization of specific bacterial structures that remain undetectable with standard microscopy methods. These techniques not only enhance the structural visualization of bacterial cells but also provide critical insights into their pathogenicity and classification. Additionally, they support diagnostic and research endeavors in microbiology by identifying key bacterial features.Capsule Staining for Virulence...
773
Flagella and Motility in Bacteria01:18

Flagella and Motility in Bacteria

1.3K
Flagella are specialized, thread-like structures that extend from a bacteria's cell envelope. They play a crucial role in motility and chemotaxis. Their structural organization and functioning exemplify sophisticated biological engineering, enabling bacterial survival and adaptability in diverse environments.Structure of the FlagellumA bacterial flagellum consists of three key components: the filament, the hook, and basal body. The filament, a long, helical structure composed of repeating...
1.3K
Fimbriae, Pili, and Axial Filaments01:28

Fimbriae, Pili, and Axial Filaments

912
Fimbriae and pili are specialized bacterial surface structures that play pivotal roles in adhesion, genetic exchange, and motility. Composed primarily of pilin protein, these hairlike appendages are crucial for bacterial survival and pathogenicity in various environments.Fimbriae: Adhesion and PathogenicityFimbriae are fine, filamentous structures measuring 2–10 nanometers in diameter and are densely distributed on the bacterial cell surface. They facilitate bacterial adhesion to abiotic...
912
Surface Appendages of Archaea01:23

Surface Appendages of Archaea

369
Archaeal surface appendages are highly specialized structures essential for environmental adaptation, encompassing roles in adhesion, biofilm formation, and motility. Among these appendages, pili and archaella stand out for their distinct morphologies and functionalities, enabling archaea to thrive in diverse and often extreme environments.Pili: Adhesion and Biofilm FormationPili are filamentous structures assembled from pilin protein subunits, primarily contributing to adhesion and biofilm...
369
Bacterial Phylum Tenericutes01:24

Bacterial Phylum Tenericutes

208
The phylum Tenericutes, which includes the single class Mollicutes, comprises bacteria that lack cell walls. The term "Mollicutes" derives from the Latin word mollis, meaning "soft." These organisms are among the smallest known and are commonly referred to as mycoplasmas due to the prominence of the genus Mycoplasma, which includes well-known human pathogens. Despite their inability to stain gram-positively (a result of their lack of cell walls), mycoplasmas are phylogenetically related to the...
208
Bacterial Phylum Planctomycetes01:26

Bacterial Phylum Planctomycetes

209
Planctomycetes are a group of morphologically distinct bacteria predominantly classified into two orders: Planctomycetales and Brocadiales. These gram-negative bacteria exhibit unique features, including division by budding and the presence of stalks or appendages. Their cells are often found in rosette arrangements, and they are notable for possessing an S-layer in their cell envelope, which is relatively uncommon among bacteria. Additionally, Planctomycetes frequently exhibit intracellular...
209

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>Staphylococcus epidermidis</i> DnaK alters biofilm formation and proteome in <i>Staphylococcus aureus</i> CIP 107093.

Frontiers in microbiology·2026
Same author

Volatile compounds as bacterial communication signals: State of art, limitations and future perspectives.

Microbiological research·2026
Same author

<i>In vitro</i> biocontrol potential of plant extract-based formulation against infection structures of <i>Phytophthora infestans</i> along with lower non-target effects.

Frontiers in microbiology·2025
Same author

Author Correction: Lifecycle of a predatory bacterium vampirizing its prey through the cell envelope and S-layer.

Nature communications·2025
Same author

The GacS/GacA two-component system strongly regulates antimicrobial competition mechanisms of <i>Pseudomonas fluorescens</i> MFE01 strain.

Journal of bacteriology·2025
Same author

Molecular Mechanisms of Bacterial Communication and Their Biocontrol.

International journal of molecular sciences·2024

Related Experiment Video

Updated: Nov 10, 2025

Preparation, Imaging, and Quantification of Bacterial Surface Motility Assays
07:35

Preparation, Imaging, and Quantification of Bacterial Surface Motility Assays

Published on: April 7, 2015

24.7K

Pseudomonas Flagella: Generalities and Specificities.

Mathilde Bouteiller1,2, Charly Dupont1,2, Yvann Bourigault1,2

  • 1LMSM, Laboratoire de Microbiologie Signaux et Microenvironnement, EA 4312, Normandy University, Université de Rouen, 27000 Evreux, France.

International Journal of Molecular Sciences
|April 3, 2021
PubMed
Summary

Bacterial flagella enable motility for colonization and virulence. This study contrasts the structure and regulation of flagella in enteric bacteria like E. coli and Pseudomonas, highlighting key differences in assembly and gene expression.

Keywords:
PseudomonasT6SSflagellaflagellar crosstalk

More Related Videos

Generation of Null Mutants to Elucidate the Role of Bacterial Glycosyltransferases in Bacterial Motility
12:29

Generation of Null Mutants to Elucidate the Role of Bacterial Glycosyltransferases in Bacterial Motility

Published on: March 11, 2022

2.5K
Investigating Flagella-Driven Motility in Escherichia coli by Applying Three Established Techniques in a Series
07:59

Investigating Flagella-Driven Motility in Escherichia coli by Applying Three Established Techniques in a Series

Published on: May 10, 2020

8.1K

Related Experiment Videos

Last Updated: Nov 10, 2025

Preparation, Imaging, and Quantification of Bacterial Surface Motility Assays
07:35

Preparation, Imaging, and Quantification of Bacterial Surface Motility Assays

Published on: April 7, 2015

24.7K
Generation of Null Mutants to Elucidate the Role of Bacterial Glycosyltransferases in Bacterial Motility
12:29

Generation of Null Mutants to Elucidate the Role of Bacterial Glycosyltransferases in Bacterial Motility

Published on: March 11, 2022

2.5K
Investigating Flagella-Driven Motility in Escherichia coli by Applying Three Established Techniques in a Series
07:59

Investigating Flagella-Driven Motility in Escherichia coli by Applying Three Established Techniques in a Series

Published on: May 10, 2020

8.1K

Area of Science:

  • Microbiology
  • Bacterial Motility
  • Molecular Biology

Background:

  • Flagella are crucial for bacterial colonization, virulence, and fitness, enabling motility in various environments.
  • Bacterial flagella consist of a membrane complex, hook, and filament, with E. coli and Salmonella being extensively studied models.
  • Significant differences exist in flagellar structure and regulation between enteric bacteria (E. coli, Salmonella) and Pseudomonas species.

Purpose of the Study:

  • To compare and contrast flagellar structure, assembly, and gene regulation between enteric bacteria and Pseudomonas.
  • To elucidate the specificities of Pseudomonas flagella, building upon knowledge from E. coli and Salmonella models.
  • To summarize energy sources for flagellar function and regulatory pathways, including potential links to the type-six secretion system.

Main Methods:

  • Comparative analysis of flagellar structure and assembly mechanisms.
  • Review of existing literature on flagellar gene expression and regulation.
  • Examination of energy requirements for flagellar production and rotation.

Main Results:

  • Gram-negative bacterial flagellar structure is highly conserved, but assembly processes differ between peritrichous (enteric) and polar (Pseudomonas) flagella.
  • Pseudomonas exhibits a four-tiered regulatory circuit for flagellar gene expression, distinct from the three-step manner in enteric bacteria.
  • General principles of flagellar fuel and rotation are summarized, with potential regulatory connections to the type-six secretion system.

Conclusions:

  • Understanding flagellar differences is key to comprehending bacterial adaptation and pathogenesis.
  • Pseudomonas flagellar systems present unique regulatory and assembly features compared to well-studied enteric models.
  • Further research into flagellar regulation may reveal novel targets for controlling bacterial infections.