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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

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Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
3.8K

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Related Experiment Video

Updated: Nov 10, 2025

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells
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A Non-Destructive, Tuneable Method to Isolate Live Cells for High-Speed AFM Analysis.

Christopher T Evans1,2, Sara J Baldock3, John G Hardy3,4

  • 1Plymouth Marine Laboratory, Plymouth PL1 3DH, UK.

Microorganisms
|April 3, 2021
PubMed
Summary

Researchers developed a novel 3D printing method for immobilizing cells for high-resolution atomic force microscopy (AFM). This technique enables detailed topographical imaging and mechanical property studies of cells like Emiliania huxleyi under physiological conditions.

Keywords:
3D printingatomic force microscopyhigh-speedimmobilizationmicroalgaemicrobemultiphoton polymerization

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Area of Science:

  • Biophysics
  • Microscopy
  • Cell Biology

Background:

  • High-resolution imaging of single cells using atomic force microscopy (AFM) requires effective cell immobilization.
  • Existing sample preparation methods may not withstand AFM forces or preserve sample integrity.

Purpose of the Study:

  • To present a flexible methodology for rapid generation of bespoke arrays for cellular AFM analysis.
  • To demonstrate the utility of multiphoton polymerization for cell immobilization.

Main Methods:

  • Utilized high-resolution three-dimensional (3D) based printing via multiphoton polymerization.
  • Generated custom arrays for immobilizing cells for AFM.
  • Employed contact mode High-Speed Atomic Force Microscopy (HS-AFM) for imaging.

Main Results:

  • Successfully generated bespoke arrays for cellular AFM analysis.
  • Acquired high-resolution topographical data from live Emiliania huxleyi cells.
  • Demonstrated continuous imaging of a single cell for over 90 minutes.

Conclusions:

  • Multiphoton polymerization offers a rapid and flexible approach for cell immobilization in AFM studies.
  • The developed methodology supports high-resolution imaging and mechanical property analysis of cells under physiological conditions.