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Angiogenesis Is Differentially Modulated by Platelet-Derived Products.

Sarah Berndt1,2,3, Gilles Carpentier4, Antoine Turzi3

  • 1Department of Plastic, Reconstructive and Aesthetic Surgery, Geneva University Hospitals, Faculty of Medicine, 1205 Geneva, Switzerland.

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Platelet-rich plasma (PRP) and PRP-hyaluronic acid (PRP-HA) effectively promote therapeutic angiogenesis in vitro. These preparations significantly enhance endothelial cell viability and microvessel network formation, suggesting their potential in regenerative medicine.

Keywords:
angiogenesisendothelial cellsplatelet-rich-plasmasecretome

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Area of Science:

  • Regenerative Medicine
  • Vascular Biology
  • Biomaterials Science

Background:

  • Platelet-derived preparations are utilized for tissue repair and regeneration.
  • Growth factors released by platelets can stimulate therapeutic angiogenesis.

Purpose of the Study:

  • To compare the in vitro effects of platelet-rich plasma (PRP), PRP-hyaluronic acid (PRP-HA), and platelet lysates (PL) on human umbilical vein endothelial cells (HUVEC).
  • To evaluate the modulation of HUVEC metabolism, viability, senescence, and angiogenic capacities by these preparations.

Main Methods:

  • In vitro study using HUVEC treated with PRP, PRP-HA, and PL at varying concentrations (5-40%).
  • Assessed biological effects including metabolism, viability, senescence, and angiogenic factor secretion.
  • Evaluated angiogenic capacities using 2D (endothelial tube formation assay) and 3D (fibrin bead assay) models.

Main Results:

  • PRP and PRP-HA stimulated HUVEC exocytosis and significantly increased cell viability.
  • All preparations inhibited HUVEC tube formation on Matrigel, but PRP enhanced network complexity.
  • PRP and PRP-HA demonstrated robust stimulation of angiogenesis in the fibrin bead assay, while PL showed a weaker response.
  • Secretome profiling identified modulation of 26 human angiogenic proteins.

Conclusions:

  • PRP and PRP-HA are effective biological tools for inducing sustained therapeutic angiogenesis.
  • These findings support the potential clinical application of PRP and PRP-HA in regenerative therapies requiring neovascularization.