Composite P(3HB-3HV)-CS Spheres for Enhanced Antibiotic Efficiency

Area of Science:

• Biomaterials Science
• Antimicrobial drug delivery
• Polymer chemistry

Background:

• Natural-derived biopolymers offer potential for advanced antimicrobial formulations.
• Developing effective and safe delivery systems for antibiotics is crucial.

Purpose of the Study:

• To develop and evaluate composite polymeric particles P(3HB-3HV)-CS as a delivery system for antibiotics.
• To assess the enhanced antimicrobial efficacy and biocompatibility of these systems.

Main Methods:

• Fabrication of stimuli-responsive composite spheres from P(3HB-3HV) and chitosan.
• Loading of bacitracin (Bac), neomycin (Neo), and kanamycin (Kan) into the spheres.
• Evaluation of antibacterial activity against Gram-positive and Gram-negative bacteria.
• Assessment of effects on macrophage proinflammatory responses and dermal fibroblast proliferation and viability.

Main Results:

• P(3HB-3HV)-CS spheres demonstrated enhanced antibiotic delivery, boosting efficiency and antibacterial susceptibility.
• Loaded spheres showed reduced proinflammatory effects on macrophages.
• These systems supported dermal fibroblast proliferation, maintained cell ultrastructure, and exhibited antioxidant activity.

Conclusions:

• Antibiotic-loaded P(3HB-3HV)-CS spheres are effective platforms for enhancing antimicrobial strategies.
• The developed systems offer biocompatible and biodegradable alternatives for drug delivery with beneficial effects on skin cells.