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Human Virome01:26

Human Virome

The human body harbors a vast and diverse viral community known as the human virome. The virome includes bacteriophages that infect bacteria, and eukaryotic viruses that infect human cells. Transient dietary and environmental viruses also contribute to this dynamic ecosystem. Estimates suggest the human body may contain on the order of 10¹³ viral particles, though abundance varies widely by body site and detection method.Comprehensive characterization of the virome has become possible only with...

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Current Developments and Challenges in Plant Viral Diagnostics: A Systematic Review.

Gajanan T Mehetre1, Vincent Vineeth Leo1, Garima Singh2

  • 1Department of Biotechnology, Mizoram University, Aizawl, Mizoram 796004, India.

Viruses
|April 3, 2021
PubMed
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Accurate plant virus identification is crucial for sustainable agriculture. This review explores next-generation sequencing (NGS) for advanced plant virus detection, diagnosis, and identification, enabling real-time, in-field analysis.

Keywords:
diagnosticsin-field analysisnanopore sequencingnext-generation sequencingomics technologiesplant virusesquasi-species

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Area of Science:

  • Plant virology
  • Agricultural science
  • Molecular diagnostics

Background:

  • Plant viral diseases cause significant global agricultural losses.
  • Emerging viral outbreaks necessitate advanced diagnostic tools.
  • Current methods like serological and nucleic acid tests have limitations.

Purpose of the Study:

  • To review recent advancements in next-generation sequencing (NGS) for plant virus detection.
  • To highlight the potential of NGS in diagnosing and identifying plant viral diseases.
  • To discuss the development of real-time, in-field diagnostic technologies.

Main Methods:

  • Review of current literature on NGS applications in plant virology.
  • Analysis of diagnostic techniques for prevalent and unknown plant viruses.
  • Discussion of portable devices for in-field diagnostics.

Main Results:

  • NGS offers high potential for simultaneous detection of multiple plant viruses.
  • Real-time, portable diagnostic technologies are emerging for in-field use.
  • NGS techniques are still in preliminary stages for routine plant viral diagnostics.

Conclusions:

  • NGS-based methods show promise for revolutionizing plant virus detection and diagnosis.
  • Development of portable, real-time tools is key for effective in-field diagnostics.
  • Further research is needed to overcome limitations and implement NGS widely in plant virology.