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Avian reovirus mRNAs are nonfunctional in infected mouse cells: translational basis for virus host-range restriction

J Benavente1, A J Shatkin

  • 1Center for Advanced Biotechnology and Medicine, Piscataway, NJ 08855-0759.

Proceedings of the National Academy of Sciences of the United States of America
|June 1, 1988
PubMed

Insights

Avian reovirus S1133 replicates in mouse cells but protein synthesis is blocked. Viral RNA is transcribed but translation initiation fails, preventing viral replication.

Area of Science:

  • Virology
  • Molecular Biology
  • Cell Biology

Background:

  • Avian reovirus S1133 infects mouse L cells.
  • Viral transcripts are produced but not translated into proteins.

Purpose of the Study:

  • To investigate the mechanism of translational block in avian reovirus-infected mouse cells.
  • To determine the stage at which viral replication is inhibited.

Main Methods:

  • Infection of mouse L cells with avian reovirus S1133.
  • Analysis of viral transcript association with polysomes.
  • Coinfection experiments with mammalian reovirus type 3.
  • In vitro translation assays using rabbit reticulocyte lysates.

Main Results:

  • Avian reovirus transcripts were found in the cytoplasm but did not associate with polysomes.
  • Protein synthesis was inhibited despite transcript production.
  • The translational block was not overcome by coinfection or chemical treatments.
  • Viral RNA from infected cells directed protein synthesis in a cell-free system.

Conclusions:

  • Avian reovirus transcription occurs in mouse cells.
  • Viral replication is blocked at the initiation of protein synthesis.
  • Mouse L cells are nonpermissive for avian reovirus replication due to a post-transcriptional defect.

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