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Lipid Peroxidation in Subretinal Fluid: Some Light on the Prognosis Factors
Francisco Bosch-Morell1,2, Enrique García-Gen1, Salvador Mérida1,2
1Departamento Ciencias Biomédicas, Biomedical Research Institute, Universidad Cardenal Herrera-CEU, CEU Universities, Av. Seminario s/n, 46113 Valencia, Spain.
Abstract:
The aim of this study was to identify a relation between the clinical characteristics and differences in lipid peroxidation in the subretinal fluid (SRF) of rhegmatogenous retinal detached patients by malondialdehyde (MDA) quantification. We collected 65 SRF samples from consecutive patients during scleral buckling surgery in rhegmatogenous retinal detachment (RRD) eyes. In addition to a complete ophthalmic evaluation, we studied the refractive status, evolution time, and the number of detached retinal quadrants to establish the extension of RRD. We studied the clinical aspects and oxidative stress and compared the characteristics among groups. We found that neither the evolution time of RRD nor the patients' age correlated with the MDA concentration in the SRF. The MDA and the protein content of the SRF increased in the patients with high myopia and with more extended RRD. Our results suggest that oxidative imbalance was important in more extended retinal detachment (RD) and in myopic eyes and should be taken into account in the managing of these cases.
Insights
Oxidative stress markers like malondialdehyde (MDA) in subretinal fluid (SRF) are elevated in rhegmatogenous retinal detachment (RRD) patients with high myopia and extensive retinal detachment. These findings highlight the role of oxidative imbalance in managing RRD.
Area of Science:
- Ophthalmology
- Biochemistry
- Cell Biology
Background:
- Rhegmatogenous retinal detachment (RRD) is a sight-threatening condition.
- Oxidative stress is implicated in the pathogenesis of various ocular diseases.
- Subretinal fluid (SRF) composition may offer insights into RRD pathophysiology.
Purpose of the Study:
- To investigate the relationship between clinical characteristics and lipid peroxidation in the SRF of RRD patients.
- To quantify malondialdehyde (MDA) levels in SRF as a marker of oxidative stress.
- To explore correlations between MDA concentration and RRD extent, duration, and patient refractive status.
Main Methods:
- Collected 65 SRF samples from RRD patients undergoing scleral buckling surgery.
- Performed ophthalmic evaluations including refractive status and RRD extent (number of detached quadrants).
- Quantified MDA and protein content in SRF samples.
Main Results:
- Neither RRD duration nor patient age correlated with SRF MDA concentration.
- MDA and protein levels in SRF were significantly higher in patients with high myopia.
- Increased SRF MDA and protein content were observed in RRD with more extensive detachment (more quadrants).
Conclusions:
- Oxidative imbalance, indicated by elevated MDA, is associated with more extensive RRD.
- Myopic eyes with RRD exhibit higher levels of oxidative stress markers in SRF.
- These findings suggest oxidative stress plays a role in RRD pathogenesis, particularly in myopic and extensive cases, warranting consideration in clinical management.
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