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Association between Venom Immunotherapy and Changes in Serum Protein-Peptide Patterns
Joanna Matysiak1, Eliza Matuszewska2, Marek L Kowalski3
1Faculty of Health Sciences, The President Stanisław Wojciechowski State University of Applied Sciences in Kalisz, 62-800 Kalisz, Poland.
Vaccines
|April 3, 2021
Summary
Venom immunotherapy (VIT) alters serum protein profiles in Hymenoptera-venom-allergic patients. Proteomic changes correlate with increased serum IgG4 levels and treatment response, offering insights into VIT mechanisms.
Area of Science:
- Immunology
- Proteomics
- Allergy Research
Background:
- Venom immunotherapy (VIT) is crucial for managing Hymenoptera venom allergies.
- Understanding VIT's molecular mechanisms is essential for optimizing treatment efficacy.
Purpose of the Study:
- To analyze serum proteomic patterns in Hymenoptera-venom-allergic patients during VIT.
- To identify protein changes associated with treatment response and serum sIgG4 levels.
Main Methods:
- Serum samples were collected from VIT patients at five time points.
- Proteomic analysis was performed to identify changes in protein expression.
- Patients were stratified into high and low responders based on serum sIgG4 levels.
Main Results:
- VIT was associated with significant changes in seven serum proteins.
- Proteomic changes and discriminative mass-to-charge ratio features increased by day 90.
- Major proteomic alterations were more pronounced in the high responder group.
Conclusions:
- Serum proteomic profiles reflect the immune response to VIT.
- Changes in protein synthesis and degradation may occur during VIT.
- Proteomic analysis can help differentiate responders and non-responders to VIT.
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