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Related Experiment Video

Updated: Nov 10, 2025

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

Published on: March 5, 2017

DNA-Mediated Stack Formation of Nanodiscs.

Madhumalar Subramanian^1,2, Charlotte Kielar¹, Satoru Tsushima¹

¹Biophysics Department, Institute of Resource Ecology, Helmholtz-Zentrum Dresden-Rossendorf, Bautzner Landstrasse 400, 01328 Dresden, Germany.

Molecules (Basel, Switzerland)

|April 3, 2021

Summary

Researchers engineered membrane mimetics called nanodiscs using scaffolding proteins. By modifying these nanodiscs with DNA, they created stacked structures, advancing membrane protein research and structural studies.

Keywords:

bionanotechnology lipid bilayer lipid protein interaction membrane protein membrane-scaffolding protein multimerization nanodisc self-assembly

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Last Updated: Nov 10, 2025

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

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Published on: March 5, 2017

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

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Co-Translational Insertion of Membrane Proteins into Preformed Nanodiscs

Co-Translational Insertion of Membrane Proteins into Preformed Nanodiscs

Published on: November 19, 2020

Area of Science:

Biochemistry
Structural Biology
Nanotechnology

Background:

Membrane-scaffolding proteins (MSPs) from apolipoprotein A-1 are used to create nanodiscs for membrane protein research.
Current research focuses on arranging membrane proteins in ordered structures for diffraction studies to overcome crystallization challenges.

Purpose of the Study:

To develop methods for forming multimers of MSP1D1-bounded nanodiscs.
To explore controlled assembly of nanodiscs for advanced structural biology applications.

Main Methods:

Engineered cysteines in MSP1D1 for chemical modification and disulfide bond formation.
Utilized fluorescent labeling, circular dichroism spectroscopy, differential light scattering, size exclusion chromatography, and transmission electron microscopy.
Employed oligonucleotide hybridization for nanodisc assembly.

Main Results:

Demonstrated successful chemical modification of MSP1D1 without compromising nanodisc integrity.
Achieved low yields of dimers and trimers via direct disulfide bond formation.
Successfully grew submicron-sized tracts of stacked nanodiscs using DNA hybridization.

Conclusions:

Engineered nanodiscs can be modified and assembled into higher-order structures.
Oligonucleotide-mediated assembly offers a promising route for creating ordered nanodisc arrays for structural studies.