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Paeoniflorin Enhances Endometrial Receptivity through Leukemia Inhibitory Factor
Hye-Rin Park1,2, Hee-Jung Choi1, Bo-Sung Kim1,2
1Korean Medical Research Center for Healthy Aging, Pusan National University, Yangsan, Gyeongsangnam-do 50612, Korea.
Biomolecules
|April 3, 2021
Summary
Paeoniflorin, derived from Paeonia lactiflora, improves embryo implantation by enhancing endometrial receptivity. This compound shows potential for treating implantation failure by boosting leukemia inhibitory factor (LIF) signaling.
Area of Science:
- Reproductive Biology
- Pharmacology
- Genomics
Background:
- Deficient endometrial receptivity is a significant challenge in assisted reproductive technology.
- Previous research indicated that Paeonia lactiflora water extract enhances endometrial receptivity by inducing leukemia inhibitory factor (LIF).
- Identifying the specific active compound responsible for this effect is crucial for therapeutic development.
Purpose of the Study:
- To identify the active compound in Paeonia lactiflora responsible for enhancing endometrial receptivity.
- To evaluate the efficacy of paeoniflorin in improving embryo implantation rates in a mouse model.
- To investigate the mechanism by which paeoniflorin enhances endometrial receptivity, focusing on LIF signaling.
Main Methods:
- Identified paeoniflorin as the major active compound in Paeonia lactiflora extract.
- Utilized a murine model of mifepristone-induced implantation failure to assess paeoniflorin's effect on embryo implantation.
- Performed in vitro experiments using human trophectoderm-derived JAr cells and endometrial Ishikawa cells to evaluate cell adhesion and LIF expression.
- Analyzed human endometrial data from the NCBI Gene Expression Omnibus (GEO) database to confirm the role of LIF signaling.
Main Results:
- Paeoniflorin significantly improved embryo implantation rates in the mifepristone-induced implantation failure model.
- In vitro, paeoniflorin enhanced the adhesion of JAr cells to Ishikawa cells, mediated by increased LIF expression.
- Analysis of human endometrial data confirmed LIF signaling as a key regulator of endometrial receptivity.
Conclusions:
- Paeoniflorin is identified as the primary active compound in Paeonia lactiflora responsible for enhancing endometrial receptivity.
- Paeoniflorin demonstrates therapeutic potential for treating endometrial implantation failure by improving embryo implantation rates.
- The mechanism involves the upregulation of LIF signaling, highlighting its importance in endometrial receptivity.
