Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Immunogenicity and Safety of Live Attenuated Varicella Vaccine in Children and Youth With Solid Organ Transplants in Canada: A Canadian Immunization Research Network Study.

Pediatric transplantation·2026
Same author

Hematologic adverse events following COVID-19 vaccination and revaccination in persons aged 12 and above: A Canadian Immunization Research Network study.

Human vaccines & immunotherapeutics·2026
Same author

Oncolytic vaccinia virus encoding constitutively active EPAC remodels the tumor microenvironment to enhance therapeutic efficacy with chemotherapy and surgery.

Journal for immunotherapy of cancer·2026
Same author

Immunoglobulin treatment and clinical outcomes: data from the Ontario Immunoglobulin Treatment program multicenter case registry.

Therapeutic advances in hematology·2025
Same author

Dengue among immunocompromised patients: a systematic review and meta-analysis.

Journal of travel medicine·2025
Same author

Mental Health and Cognitive Outcomes in Patients Six Months After Testing Positive Compared with Matched Patients Testing Negative for COVID-19 in a Non-Hospitalized Sample: A Matched Retrospective Cohort Study.

International journal of environmental research and public health·2025

Related Experiment Video

Updated: Nov 10, 2025

Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19
08:48

Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19

Published on: February 16, 2022

3.1K

A High-Throughput NanoBiT-Based Serological Assay Detects SARS-CoV-2 Seroconversion.

Taha Azad1,2, Reza Rezaei1,2, Ragunath Singaravelu1,2

  • 1Ottawa Hospital Research Institute, Ottawa, ON K1H 8L6, Canada.

Nanomaterials (Basel, Switzerland)
|April 3, 2021
PubMed
Summary

A new NanoBiT-based assay rapidly detects SARS-CoV-2 antibodies in recovered COVID-19 patients and vaccinated individuals. This high-throughput method offers a stable and sensitive solution for global serosurveys.

Keywords:
COVID-19NanoBiTSARS-CoV-2serological assay

More Related Videos

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System
07:08

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

Published on: April 6, 2021

5.2K
Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
10:25

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Published on: June 5, 2021

5.0K

Related Experiment Videos

Last Updated: Nov 10, 2025

Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19
08:48

Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19

Published on: February 16, 2022

3.1K
A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System
07:08

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

Published on: April 6, 2021

5.2K
Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
10:25

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Published on: June 5, 2021

5.0K

Area of Science:

  • Immunology
  • Biotechnology
  • Epidemiology

Background:

  • High-throughput detection of SARS-CoV-2 antibodies is crucial for managing the pandemic.
  • Current serological assays often lack the speed, stability, sensitivity, and specificity for global use.
  • There is an urgent need for improved methods for seroconversion screening.

Purpose of the Study:

  • To develop and validate a novel, high-throughput NanoBiT-based serological assay.
  • To enable fast and sensitive detection of SARS-CoV-2 receptor-binding domain (RBD)-specific antibodies.
  • To provide a stable and accessible tool for worldwide seroepidemiologic surveillance.

Main Methods:

  • Development of a NanoBiT-based reporter system for antibody detection.
  • High-throughput assay format capable of processing 384 samples per hour.
  • Assessment of assay performance using minimal serum (5 μL) or antibody (10 ng) quantities.
  • Evaluation of NanoBiT reporter stability across a range of temperatures (4-42 °C) and pH (4-12).

Main Results:

  • The NanoBiT assay demonstrated fast and sensitive detection of SARS-CoV-2 RBD-specific antibodies.
  • The assay is suitable for high-throughput screening, processing 384 samples/hour.
  • The NanoBiT reporter exhibited stability under various environmental conditions, indicating robustness for global deployment.
  • The assay requires minimal sample volumes, enhancing accessibility.

Conclusions:

  • The developed NanoBiT-based assay is a rapid, sensitive, and high-throughput method for detecting SARS-CoV-2 antibodies.
  • Its stability and accessibility make it a valuable tool for post-vaccination seroconversion screening and global serosurveys.
  • This assay offers a potentially cost-effective solution for monitoring population immunity.