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Updated: Nov 10, 2025

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
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RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

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Long Non-Coding RNA CRYBG3 Promotes Lung Cancer Metastasis via Activating the eEF1A1/MDM2/MTBP Axis

Anqing Wu1,2, Jiaxin Tang1,2, Ziyang Guo1,2

  • 1State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, China.

International Journal of Molecular Sciences
|April 3, 2021
PubMed

Insights

A novel long non-coding RNA (lncRNA), LNC CRYBG3, promotes non-small cell lung cancer (NSCLC) metastasis by regulating the eEF1A1/MDM2/MTBP/ACTN4 pathway. This discovery offers a potential new therapeutic target for NSCLC treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Distant tumor metastases significantly impede non-small cell lung cancer (NSCLC) treatment and patient outcomes.
  • Long non-coding RNAs (lncRNAs) are increasingly recognized as key regulators of metastasis in lung cancer.

Purpose of the Study:

  • To investigate the role and underlying mechanisms of the novel long non-coding RNA (lncRNA) LNC CRYBG3 in regulating NSCLC metastasis.

Main Methods:

  • Quantitative analysis of LNC CRYBG3 expression in NSCLC cells versus normal tissues.
  • In vitro experiments involving overexpression of LNC CRYBG3 in HCC827 cells to assess metastatic potential and protein expression.
  • In vivo metastasis assays.
  • Mechanistic studies to elucidate the interaction of LNC CRYBG3 with eEF1A1, MDM2, MTBP, and ACTN4.

Main Results:

  • LNC CRYBG3 was found to be upregulated in NSCLC cells and correlated with metastatic ability.
  • Overexpression of LNC CRYBG3 enhanced metastasis and increased Snail and Vimentin protein levels in HCC827 cells, both in vitro and in vivo.
  • LNC CRYBG3 directly binds to eEF1A1, promoting nuclear translocation and enhancing MDM2 transcription. This leads to MDM2/MTBP complex formation, disrupting MTBP/ACTN4 binding and increasing cell migration.

Conclusions:

  • The LNC CRYBG3/eEF1A1/MDM2/MTBP axis represents a newly identified signaling pathway crucial for regulating tumor metastasis in NSCLC.
  • LNC CRYBG3 emerges as a potential therapeutic target for managing NSCLC metastasis.
Keywords:
MDM2NSCLCeEF1A1long non-coding RNAmetastasis

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