Supercritical-CO₂ extraction, identification and quantification of polyprenol as a bioactive ingredient from Irish...

This study ascertained the accumulation of polyprenol from four Irish conifer species Picea sitchensis, Cedrus atlantica 'Glauca', Pinus sylvestris and Taxus baccata and one flowering tree Cotoneaster hybrida using supercritical fluid extraction with carbon dioxide (SFE-CO₂) and solvent extraction. The effects of SFE-CO₂ parameters such as temperature (ranged from 40 to 70 [Formula: see text]), pressure (ranged from 100 to 350 bars) and dynamic time (from 70 min to 7 h) were analysed on the extraction efficiency of polyprenol. Qualitative and quantitative analysis of polyprenol was examined using high-performance liquid chromatography. Results showed that P. sylvestris accumulated the highest polyprenol yield of 14.00  ± [Formula: see text]mg g^-1 DW when extracted with hexane:acetone (1:1 v/v). However, with SFE-CO₂ conditions of 200 bars, 70 [Formula: see text], 7 h, with absolute ethanol as a cosolvent with a flow rate of 0.05 ml min^-1, P. sitchensis accumulated the highest polyprenol yield of 6.35 ± [Formula: see text] mg g^-1DW. This study emphasised the potential application of SFE-CO₂ in the extraction of polyprenol as an environmentally friendly method to be used in pharmaceutical and food industries.