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Updated: Nov 10, 2025
Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226
Published on: May 10, 2022
Th2 cell-derived histamine is involved in nasal Th2 infiltration in mice
Naruhito Iwasaki1,2, Seigo Terawaki3, Kouhei Shimizu3
1Department of Pathobiochemistry, Graduate School of Medicine, Osaka City University, Osaka, Japan. m2071288@med.osaka-cu.ac.jp.
Objective:
Histamine derived from mast cells and basophils plays important roles in inducing allergic symptoms. Although T cells also produce histamine, the involvement of the histamine produced from T cells has remained enigmatic. We sought to reveal the roles of T helper 2 (Th2) cell-derived histamine in nasal allergic disorders.
Methods:
The histamine production from Th2 cells was measured by EIA. The mRNA expression of histidine decarboxylase (HDC) was measured by real-time PCR. To investigate the roles of Th2 cell-derived histamine in vivo, we analyzed an antigen-specific Th2 cell transfer mouse model.
Results:
Th2 cells produced histamine by T cell receptor stimulation, and these properties were specific for Th2 cells, but not Th1 cells and naïve CD4 T cells. The histamine produced from Th2 cells was involved in the infiltrations of Th2 cells in response to antigen exposure.
Conclusion:
These results suggest that Th2 cell-derived histamine play important roles in nasal allergic disorders.
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