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Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
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Upconversion nanoparticles-based FRET system for sensitive detection of Staphylococcus aureus.

Qin Ouyang1, Yongcun Yang1, Shujat Ali2

  • 1School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|April 3, 2021
PubMed
Summary

A new aptasensor detects Staphylococcus aureus (S. aureus) in food using upconversion fluorescence. This rapid and sensitive method offers accurate bacterial quantification for improved food safety.

Keywords:
AptamerFluorescence resonance energy transferGold nanoparticlesStaphylococcus aureusUpconversion nanoparticles

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Area of Science:

  • Biotechnology
  • Food Safety
  • Nanotechnology

Background:

  • Staphylococcus aureus (S. aureus) poses a significant threat to food safety.
  • Accurate detection of S. aureus is crucial for preventing foodborne illnesses.

Purpose of the Study:

  • To develop a rapid, sensitive, and specific aptasensor for S. aureus detection in food.
  • To utilize upconversion fluorescence resonance energy transfer (FRET) for enhanced detection capabilities.

Main Methods:

  • Fabrication of an aptasensor using aptamer-functionalized gold nanoparticles (AuNPs-aptamers) and cDNA-modified upconversion nanoparticles (UCNPs-cDNA).
  • Detection principle based on fluorescence quenching and recovery via S. aureus-aptamer interaction.
  • Optimization of assay conditions for maximal sensitivity and specificity.

Main Results:

  • The aptasensor demonstrated a linear response between fluorescence intensity and S. aureus concentration (47–4.7 × 10^7 CFU/mL, R² = 0.9904).
  • Achieved a low detection limit of 10.7 CFU/mL for S. aureus.
  • Validation against the standard plate count method confirmed the biosensor's precision and accuracy.

Conclusions:

  • The developed upconversion FRET aptasensor provides a promising tool for rapid and sensitive quantification of S. aureus in food samples.
  • This method has significant potential for application in routine food safety monitoring.
  • The aptasensor offers a sensitive and specific alternative for bacterial detection in complex food matrices.