Related Experiment Videos
[Primary Study on Chronic Myeloid Leukemia in NCG Mice from Qinba Mushroom]
Ming-Xia Cheng1, Jing Qi2, Lian-Ping Zhao3
1Department of Hematology, Gansu Provincial Hospital;Lanzhou 730000, Gansu Province, China.
Objective:
The present study was to evaluate the anti-tumor effects of acidic RNA protein complex (FA-2-b-β) extracted from the wild edible Qinba mushroom in inducing of apoptosis and immunoregulation of tumor cell.
Methods:
Cell proliferation inducing rate of FA-2-b-β to K562 cell was measured using CCK-8. Apoptosis rate was detected by using flow cytometry. Chronic myeloid leukemia model was developed by tail vein injection/subcutaneous inoculation of K562 cells in NCG mice. The tumor burden of mice was observed. The general condition of the mice was monitored twice daily. The peripherivcal full blood counts of mice was tested daily. RT-qPCR and Western blot was FA-2-b-β performed to determine involvement of apoptotic-related gene and protenin, Immunofluorescence and immunohistochemistry was used to detected the expression of CD3, CD4 and CD8.
Results:
The proliferation and apoptosis of K562 cell could be inhibitied and induced by FA-2-b-β, there was 100% successful in the tumor formation in vivo, after treated by drug for 21 days there were significantly increased peripheral leucocytes, but decreased hemoglobin of mice treated by FA-2-b-β as compared with those in control group. The CD3, CD4 and CD8 showed positive in mice, and the propotation was imbalance, but it showed reserved after treated by FA-2-b-β.
Conclusion:
FA-2-b-β is strong anti-leukemia effect in vitro and in vivo, suggesting the traditional Chinese medicine maybe contribute to the anti-cancer and immunoregulation research.
Insights
Acidic RNA protein complex (FA-2-b-β) from Qinba mushroom shows anti-leukemia effects by inducing apoptosis and regulating immune cells. This traditional Chinese medicine offers potential for cancer treatment and immunotherapy research.
Area of Science:
- Biochemistry
- Immunology
- Pharmacology
Background:
- The Qinba mushroom yields an acidic RNA protein complex, FA-2-b-β.
- This complex is investigated for its potential anti-tumor properties.
Purpose of the Study:
- To evaluate the anti-tumor effects of FA-2-b-β.
- To assess its role in inducing apoptosis and immunoregulation in tumor cells.
Main Methods:
- Cell proliferation and apoptosis assays (CCK-8, flow cytometry) on K562 cells.
- In vivo studies using a chronic myeloid leukemia mouse model.
- Analysis of peripheral blood counts, tumor burden, and immune cell markers (CD3, CD4, CD8) via RT-qPCR, Western blot, immunofluorescence, and immunohistochemistry.
Main Results:
- FA-2-b-β inhibited K562 cell proliferation and induced apoptosis in vitro.
- In vivo, FA-2-b-β treatment led to increased leukocytes and altered hemoglobin levels.
- Immune cell analysis revealed a restoration of balance in CD3, CD4, and CD8 expression post-treatment.
Conclusions:
- FA-2-b-β exhibits significant anti-leukemia activity both in vitro and in vivo.
- The findings suggest FA-2-b-β's potential as a therapeutic agent in cancer and for immunoregulation research.