Quantitation of thiothixene in plasma by high-performance thin-layer chromatography and fluorometric detection

A specific and sensitive assay procedure to measure thiothixene (Navane) in plasma has been developed and used to measure plasma concentrations in patients receiving thiothixene. The procedure involves in situ fluorescent detection after separation by high-performance thin-layer chromatography. Fluorescent detection permits a limit of detectability of approximately 0.1 ng/ml in plasma and the coefficient of variation is less than 6% at 2 ng/ml. Thirty samples may be processed through the entire procedure in less than 6-h period and up to 60 samples may be simultaneously spotted and chromatographed with a larger-capacity spotter and plate. Plasma levels (n = 62) drawn 10-12 h after dosage ranged from 0 to 42 ng/ml from dosages of 4-100 mg/day.