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Interrogating immune cells and cancer with CRISPR-Cas9
Frank A Buquicchio1, Ansuman T Satpathy2
1Program in Immunology, Stanford University School of Medicine, Stanford, CA, USA.
Trends in Immunology
|April 4, 2021
Summary
CRISPR-Cas9 gene editing advances immunology research and cancer immunotherapy development. This review details CRISPR-Cas9 applications for discovering immune system regulators and designing novel cancer treatments.
Area of Science:
- Immunology
- Genetics
- Cancer Research
Background:
- CRISPR-Cas9 technology revolutionizes genetic pathway studies.
- Recent adaptations target immune cells, boosting functional genomics.
- This accelerates development of cellular immunotherapies for cancer.
Purpose of the Study:
- To review recent CRISPR-Cas9 developments.
- To discuss their application in immune system research.
- To highlight their role in immuno-oncology.
Main Methods:
- Review of recent CRISPR-Cas9 technology advancements.
- Analysis of CRISPR-Cas9 applications in immune cell research.
- Exploration of CRISPR-Cas9-based screens in immunology.
Main Results:
- CRISPR-Cas9 has transformed the study of cellular differentiation and function.
- Adapted CRISPR-Cas9 methods accelerate functional genomics in immunology.
- New avenues for cancer cellular immunotherapy design are enabled.
Conclusions:
- CRISPR-Cas9 is a powerful tool for discovering and manipulating immune system regulators.
- This technology is crucial for advancing immuno-oncology.
- The review serves as a resource for CRISPR-Cas9 based screens in immunology and cancer therapy.
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