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Updated: Nov 10, 2025
A Pipeline to Characterize Structural Heart Defects in the Fetal Mouse
Published on: December 16, 2022
MORPHOLOGICAL FEATURES OF RATS' HEARTS AFTER INTRAFETAL INJECTION OF DEXAMETHASONE
Olena A Hryhorieva1, Arthur V Chernyavskiy1, Yuriy Yo Guminskiy2
1ZAPORIZHZHIA STATE MEDICAL UNIVERSITY, ZAPORIZHZHIA, UKRAINE.
Objective:
The aim: Is to study the morphological features of rats' hearts after prenatal administration of glucocorticoids.
Patients And Methods:
Materials and methods: In this study we used histological, immunohistochemical, electron-microscopic and statistical research methods.
Results:
Results: It is found that at 30th day after birth in rats after intrafetal introduction of dexamethasone in myocardium a relative area occupied by arterial vessels is significantly smaller in comparison with control. Absolute and relative number of Ki-67+-cardiomyocytes in the myocardium of experimental rats is reduced throughout the second week after birth and is significantly less compared to the control group. In the nuclei of cardiomyocytes of experimental rats is rendered the greater amount of heterochromatin in comparison with cardiomyocytes of the control group where euchromatin prevails.
Conclusion:
Conclusions: After intrafetal injection of dexametazone changes in dynamics and significantly smaller index of relative area occupied by arterial vessels in ventricular myocardium at the 30th day after birth are observed; the absolute and relative number of Ki-67+ -cardiomyocytes in myocardium decreases during the second week after birth and is significantly lower compared to the control group; in the nuclei of cardiomyocytes of experimental rats a greater amount of heterochromatin is visualized, and in cardiomyocytes of the control group - euchromatin.
