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Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

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Published on: July 6, 2016

Cyanide-sensitive fluorescent probes.

Ramachandram Badugu¹, Joseph R Lakowicz¹, Chris D Geddes^1,2

¹Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, Medical Biotechnology Center, University of Maryland School of Medicine, 725 West Lombard St, Baltimore, MD 21201, USA.

Dyes and Pigments : an International Journal

|April 5, 2021

Summary

This summary is machine-generated.

Boronic acid fluorophores can detect aqueous cyanide, a finding applicable to many probes. This generic sensing mechanism enables diverse fluorescence-based detection methods for cyanide.

Keywords:

Boronic acid Colorimetric response Cyanide sensing Physiological safeguard Poison Ratiometric fluorescence sensing

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Area of Science:

Analytical Chemistry
Biochemistry
Materials Science

Background:

Boronic acid fluorophores are crucial for sugar determination.
Previous studies demonstrated their utility in sensing aqueous cyanide at physiological levels.
A need exists for a universal sensing mechanism for cyanide detection.

Purpose of the Study:

To investigate the generic response of boronic acid fluorophores to aqueous cyanide.
To establish a broad applicability of this sensing mechanism for cyanide detection.
To explore various fluorescence modalities for cyanide sensing.

Main Methods:

Characterization of boronic acid-containing fluorophores.
Testing probe response to aqueous cyanide solutions.
Analysis of fluorescence changes (intensity, lifetime, ratiometric, polarization, modulation).

Main Results:

The cyanide sensing mechanism is generic to the boronic acid moiety.
This mechanism is not limited to specific, recently reported probes.
A wide range of cyanide-sensitive probes can be developed.

Conclusions:

Boronic acid fluorophores offer a versatile platform for cyanide sensing.
The generic nature of the mechanism expands the scope of fluorescence-based cyanide detection.
Multiple fluorescence sensing modalities can be employed for cyanide detection.