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Updated: Nov 10, 2025

In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability
Published on: October 19, 2018
Mucosal Epithelial Jak Kinases in Health and Diseases
Narendra Kumar1, Longxiang Kuang1, Ryan Villa1
1Department of Pharmaceutical Sciences, ILR College of Pharmacy, Texas A&M Health Science Center, Kingsville TX 78363, USA.
Janus kinases (Jaks), particularly Jak3, are crucial for both immune and epithelial cell functions. Dysregulation of Jaks is linked to diseases, highlighting their therapeutic potential in epithelial cancers and inflammatory conditions.
Area of Science:
- Immunology
- Cell Biology
- Molecular Biology
Background:
- Janus kinases (Jaks) are nonreceptor tyrosine kinases with four members: Jak1, Jak2, Jak3, and Tyk2.
- While critical in immune cells, Jaks also regulate nonimmune cell functions, including epithelial cells.
Purpose of the Study:
- This review highlights the role of epithelial Jaks in disease pathogenesis.
- It focuses on Janus kinase 3 (Jak3) in intestinal epithelial cells (IECs) and its implications in gastrointestinal diseases.
Main Methods:
- Literature review of studies on Janus kinases, with a focus on Jak3.
- Analysis of Jak3's role in epithelial-mesenchymal transition, cell survival, growth, differentiation, and disease development.
Main Results:
- Jak3 is expressed in both immune and intestinal epithelial cells (IECs).
- Mutations in Jak3 cause severe combined immunodeficiency (SCID) or cancer.
- Jak3 inhibition (e.g., CP-690550) shows therapeutic effects but also adverse effects.
Conclusions:
- Epithelial Jaks, especially Jak3, are significant in understanding diseases like inflammatory bowel disease, obesity, and epithelial cancers.
- Further research into Jak3's gastrointestinal functions may reveal novel therapeutic targets for dysfunctional IECs.
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