Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Coordination of Gene Expression Processes in Bacteria01:29

Coordination of Gene Expression Processes in Bacteria

332
The DNA replication, transcription, and translation processes are intricately coupled in bacteria, allowing efficient gene expression and rapid protein synthesis. While this physical and functional coordination is advantageous, it introduces challenges that bacteria overcome through specific regulatory mechanisms.Coupling of Replication, Transcription, and TranslationThe coupling of replication, transcription, and translation is a hallmark of bacterial gene expression. As the replisome unwinds...
332
Ribosome Profiling02:24

Ribosome Profiling

3.8K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.8K
Translation in Prokaryotes01:29

Translation in Prokaryotes

676
Prokaryote translation is a complex, highly coordinated process that converts genetic information from mRNA into functional proteins. It involves three stages: initiation, elongation, and termination, each facilitated by specific molecular components.Initiation of TranslationThe process begins with the assembly of the ribosomal subunits and initiation factors on the mRNA. In bacteria, the 30S ribosomal subunit recognizes the Shine-Dalgarno sequence in the mRNA, a conserved region upstream of...
676
Ribosomes01:27

Ribosomes

9.2K
Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome...
9.2K
Ribosomes01:27

Ribosomes

72.6K
Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome...
72.6K
Directing Proteins to the Rough Endoplasmic Reticulum01:34

Directing Proteins to the Rough Endoplasmic Reticulum

14.4K
The organelle-specific signaling sequences direct proteins synthesized in the cytosol to their final destination like ER, mitochondria, peroxisomes, etc. Some of the proteins directed to ER are then trafficked via vesicles to other organelles within the cell or the extracellular environment through the Golgi complex. For example, the rough ER synthesizes soluble proteins for transportation to the lysosomes or secretion out of the cell. It can also synthesize transmembrane proteins that can...
14.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Patient-derived tissue slice cultures from endoscopic biopsies as a translational ex vivo model for inflammatory bowel diseases.

Clinical and experimental medicine·2026
Same author

PPARγ controls I3C-mediated anti-inflammatory effects through the NF-κB/IL-6 pathway on gut inflammation models.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same author

Flipping antimicrobial peptides in the exit tunnel of the bacterial ribosome.

Nature communications·2026
Same author

A natural depsipeptide antibiotic binds the E-site of the bacterial ribosome.

Nature·2026
Same author

Diverse mechanisms of translation arrest by a Clostridia ribosome stalling peptide CliM.

Nature communications·2026
Same author

Endothelial dysfunction and metabolic biomarkers in post-COVID-19 syndrome.

Scientific reports·2026

Related Experiment Video

Updated: Nov 10, 2025

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing
12:05

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing

Published on: August 7, 2021

8.7K

Ribosome Rescue Pathways in Bacteria.

Claudia Müller1, Caillan Crowe-McAuliffe1, Daniel N Wilson1

  • 1Institute for Biochemistry and Molecular Biology, University of Hamburg, Hamburg, Germany.

Frontiers in Microbiology
|April 5, 2021
PubMed
Summary
This summary is machine-generated.

Bacteria use diverse ribosomal rescue pathways to survive stalled protein synthesis. These systems remove stalled ribosomes and damaged mRNAs, offering potential antimicrobial targets.

Keywords:
ArfAArfBRqcHSmpBpeptidyl-tRNA drop-offribosome rescueribosome-associated quality controltmRNA

More Related Videos

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses
11:19

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses

Published on: February 25, 2011

20.1K
De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
08:23

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Published on: February 18, 2022

3.9K

Related Experiment Videos

Last Updated: Nov 10, 2025

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing
12:05

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing

Published on: August 7, 2021

8.7K
Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses
11:19

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses

Published on: February 25, 2011

20.1K
De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
08:23

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Published on: February 18, 2022

3.9K

Area of Science:

  • Molecular Biology
  • Microbiology
  • Genetics

Background:

  • Ribosomes can stall on damaged or truncated mRNAs during protein synthesis, necessitating rescue for cell survival.
  • Bacteria possess multiple pathways to rescue stalled ribosomes and degrade aberrant mRNA-polypeptide complexes.
  • Understanding these rescue mechanisms is crucial for bacterial viability and potential therapeutic interventions.

Purpose of the Study:

  • To review recent advancements in understanding bacterial ribosomal rescue systems.
  • To highlight the diversity of rescue pathways, including trans-translation and alternative factors.
  • To discuss ribosome-associated quality control mechanisms and their implications.

Main Methods:

  • Literature review of recent developments in bacterial ribosomal rescue.
  • Analysis of mechanistic insights into various rescue pathways.
  • Discussion of ribosome-associated quality control factors RqcH and RqcP.

Main Results:

  • Detailed overview of drop-off, trans-translation (tmRNA-SmpB), ArfA/ArfB, and Bacillus ribosome rescue factor A pathways.
  • Exploration of ribosome-associated quality control mechanisms (RqcH, RqcP) in specific bacterial lineages.
  • Emphasis on the conserved importance of ribosomal rescue for bacterial survival.

Conclusions:

  • Bacterial ribosomal rescue systems are essential for survival against aberrant protein synthesis.
  • These pathways represent promising targets for developing novel antimicrobial agents against drug-resistant bacteria.
  • Further research into these mechanisms can unlock new strategies for combating bacterial infections.