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Immune Negative Regulator TIPE2 Inhibits Cervical Squamous Cancer Progression Through Erk1/2 Signaling

Li-Qiong Huang1, Bo Zheng1, Yi He1

  • 1Department of Obstetrics and Gynecology, Xianning Central Hospital, The First Affiliated Hospital of Hubei University of Science and Technology, 228 Jingui Road, Xianning 437100, China.

Open Life Sciences
|April 5, 2021
PubMed

Insights

Tumor necrosis factor-α-induced protein-8-like 2 (TIPE2) inhibits cervical cancer cell growth and spread. Higher TIPE2 levels in tumors correlate with less metastasis, suggesting TIPE2

Area of Science:

  • Oncology
  • Immunology
  • Molecular Biology

Background:

  • Tumor necrosis factor-α-induced protein-8-like 2 (TIPE2) is an immune negative regulator.
  • The role of TIPE2 in cervical squamous cancer progression requires further investigation.

Purpose of the Study:

  • To investigate the function of TIPE2 in cervical squamous cancer cell proliferation and invasion.
  • To explore the association between TIPE2 expression and clinicopathological features, including lymphatic metastasis.
  • To elucidate the mechanism by which TIPE2 affects the extracellular signal-regulated kinase (Erk) signaling pathway.

Main Methods:

  • Western blot and immunohistochemistry (IHC) were used to assess TIPE2 expression in cervical cancer tissues and cell lines (SiHa, C33A).
  • Cell lines were transfected with a TIPE2-encoding plasmid to study its effects on proliferation, migration, and invasion.
  • Western blot analysis was performed to examine the phosphorylation status of Erk1/2 and MEK1/2 signaling pathway components.

Main Results:

  • TIPE2 expression was significantly higher in cervical cancer tissues compared to normal tissues.
  • Higher IHC scores for TIPE2 in tumors were negatively correlated with lymphatic metastasis.
  • Overexpression of TIPE2 suppressed cervical cancer cell proliferation, migration, and invasion in vitro.
  • TIPE2 overexpression reduced the phosphorylation of Erk1/2 and MEK1/2, indicating inhibition of the Erk signaling pathway.

Conclusions:

  • TIPE2 expression is negatively associated with the development and progression of cervical squamous cancer.
  • TIPE2 acts as an inhibitory factor for cervical squamous cancer cell proliferation and invasion.
  • The inhibitory effects of TIPE2 may be mediated through the suppression of the Erk signaling pathway.
Keywords:
cervical squamous cancerinvasion, extracellular signal-regulated kinaseproliferationtumor necrosis factor-α-induced protein-8-like 2

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