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Lnc-PICSAR contributes to cisplatin resistance by miR-485-5p/REV3L axis in cutaneous squamous cell carcinoma

Dan Wang1, Xiaoqiang Zhou2, Jing Yin3

  • 1Plastic Surgery Center, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, 214000, China.

Open Life Sciences
|April 5, 2021
PubMed
Abstract

Insights

Long noncoding RNA PICSAR promotes cisplatin resistance in cutaneous squamous cell carcinoma by regulating the miR-485-5p/REV3L pathway. Exosomal PICSAR may also contribute to drug resistance in CSCC patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Dysregulation of long noncoding RNAs (lncRNAs) is implicated in cancer drug resistance.
  • Cutaneous squamous cell carcinoma (CSCC) exhibits resistance to cisplatin (DDP), a common chemotherapeutic agent.

Purpose of the Study:

  • To investigate the role of lncRNA PICSAR in DDP resistance in CSCC.
  • To elucidate the molecular mechanism underlying PICSAR's function in DDP-resistant CSCC.

Main Methods:

  • Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot assays.
  • Cell counting kit-8 (CCK-8), transwell, and dual-luciferase reporter assays.
  • Murine xenograft models and exosome analysis (TEM, NTA).

Main Results:

  • Lnc-PICSAR expression was elevated in DDP-resistant CSCC cells and patient-derived exosomes.
  • Silencing lnc-PICSAR reduced cell viability, DDP resistance, migration, and invasion.
  • Lnc-PICSAR sponges miR-485-5p, leading to increased REV3L expression, which promotes CSCC progression and DDP resistance.
  • Lnc-PICSAR knockdown inhibited DDP resistance in vivo.

Conclusions:

  • Lnc-PICSAR enhances DDP resistance in CSCC through the miR-485-5p/REV3L axis.
  • Exosome-mediated lnc-PICSAR may play a role in CSCC drug resistance, suggesting potential diagnostic or therapeutic targets.
Keywords:
CSCCDDP resistanceREV3Lexosomelnc-PICSARmiR-485-5p

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