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Atg27p localization is clathrin- and Ent3p/5p-dependent.
Verónica A Segarra1, Anupam Sharma2, Sandra K Lemmon3
1Department of Biology, High Point University, High Point, NC, USA 27268.
Micropublication Biology
|April 5, 2021
Summary
The autophagy protein Atg27p localizes to specific cellular structures. Its localization depends on clathrin and the Ent3p/5p adaptors, revealing new insights into cellular transport mechanisms.
Area of Science:
- Cell Biology
- Molecular Biology
- Autophagy Research
Background:
- The autophagy-related protein Atg27p is known to localize to the pre-autophagosomal structure (PAS) and various organelles, including the late Golgi, vacuolar membrane, and endosome.
- Atg27p's vacuolar membrane localization is linked to its C-terminal tyrosine sorting motif and the AP-3 adaptor.
- Previous observations indicate Atg27p can be present in clathrin-coated vesicles.
Purpose of the Study:
- To investigate the role of clathrin and its associated cargo adaptors in the cellular localization of Atg27p.
- To determine if Atg27p's intracellular trafficking is mediated by clathrin-dependent pathways.
Main Methods:
- Analysis of Atg27p localization in yeast cells under conditions affecting clathrin and adaptor protein function.
- Utilizing genetic and cell biological techniques to assess the dependency of Atg27p localization on clathrin and specific adaptors like Ent3p/5p.
Main Results:
- Atg27p's localization within the cell is dependent on the presence and function of clathrin.
- The study identified a specific dependency of Atg27p localization on the cargo adaptors Ent3p and Ent5p (Ent3p/5p).
Conclusions:
- Cellular localization of Atg27p is regulated by clathrin-mediated trafficking pathways.
- Ent3p/5p adaptors play a crucial role in directing Atg27p to its correct cellular compartments, likely via clathrin-coated vesicles.
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