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Related Experiment Video

Updated: Nov 10, 2025

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Precisely Encoded Barcodes through the Structure-Fluorescence Combinational Strategy: A Flexible, Robust, and

Yao Wang1, Cang Chen1, Jing He1

  • 1Shanghai Jiao Tong University Affiliated Sixth People's Hospital, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, P. R. China.

Small (Weinheim an Der Bergstrasse, Germany)
|April 5, 2021
PubMed
Summary

Researchers developed novel microbeads for high-throughput detection, achieving an unprecedented 300-plex capacity using structural and fluorescence encoding. This advancement offers a robust platform for multiplexed biodetection, including tumor marker assays.

Keywords:
barcodesmesoporous microbeadsmultiplexed detectionstructure-fluorescenceultrahigh encoding capacities

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Area of Science:

  • Nanotechnology
  • Biotechnology
  • Analytical Chemistry

Background:

  • Suspension array technology requires microbead barcodes with high encoding capacity for multiplexed detection.
  • Existing methods face limitations in achieving ultrahigh encoding levels.

Purpose of the Study:

  • To develop a novel structure-fluorescence combinational encoding strategy for microbead barcodes.
  • To establish a barcode library with ultrahigh encoding capacities for high-throughput multiplexed detection.

Main Methods:

  • Utilized the transformability of mesoporous microbead structural parameters (porosity, matrix component) into scattering signals.
  • Combined structural encoding with two fluorescent elements: fluorescein isothiocyanate isomer I (FITC) and quantum dots (QDs).
  • Constructed barcodes with FITC encapsulated within mesopores, magnetic nanoparticles, and surface-immobilized QDs.

Main Results:

  • Achieved an ultrahigh encoding capacity of 300, the highest recorded for single excitation laser-based systems.
  • Demonstrated superparamagnetism in the designed microbead barcodes.
  • Successfully evaluated a ten-plexed tumor markers bioassay using the tailored barcodes, confirming feasibility and effectiveness.

Conclusions:

  • The proposed structure-fluorescence combinational encoding strategy significantly enhances barcode library capacity.
  • The developed microbead barcodes offer a promising and robust platform for practical multiplexed biodetection.
  • This technology represents a breakthrough in achieving ultrahigh multiplexing for diagnostic applications.