Related Experiment Video
Updated: Sep 23, 2026
Injections of Lipopolysaccharide into Mice to Mimic Entrance of Microbial-derived Products After Intestinal Barrier Breach
Published on: May 2, 2018
Intestinal microbiota and antibiotic-associated acute gastrointestinal injury in sepsis mice
1Department of Critical Care Medicine, The First Affiliated Hospital of Harbin Medical University, Harbin Medical University, Harbin 150001, Heilongjiang, China.
Background:
To investigate the changes of intestinal microbiota and metabolites in sepsis mice with acute gastrointestinal injury before and after the use of antibiotics, and to explore the possible effects of these changes on the body.
Methods:
Twenty-four 6-8-w-old SPF-grade C57BL/6J male mice were selected, and the mice were randomly divided into three groups. The mice were treated by tail vein injection for 3 days. The intestinal motility of mice after administration was detected. The mice feces were collected for 16S rRNA and Untargeted metabonomics detection.
Results:
The use of antibiotics in sepsis mice can change the composition of intestinal microbiota and metabolites. LD3, AD3 and LAD3 samples had significant differences in bacterial species. Desulfovibrio was the species with a significant difference in LAD3. In addition, we found that the composition of those intestinal microbiota were correlated with changes in intestinal motility. The untargeted metabolomics analysis showed that the fecal metabolites of LD3 and LAD3 samples were significantly different. In addition to the basic metabolites, Benzoic acid and 4-Hydroxybenzoic acid were also found, and Desulfovibrio was associated with them.
Conclusions:
The use of antibiotics in sepsis mice can lead to changes in the intestinal microbiota and metabolite levels, which may be related to the severity of acute gastrointestinal injury in sepsis mice. Inhibiting Desulfovibrio in the intestine and using Benzoic acid and 4-Hydroxybenzoic acid as a marker for the production of Desulfovibrio may reduce the inflammatory degree of acute gastrointestinal injury in sepsis.
Insights
Antibiotics alter gut microbiota and metabolites in sepsis mice, impacting intestinal motility. Targeting Desulfovibrio and using benzoic acid may reduce acute gastrointestinal injury.
Area of Science:
- Microbiology
- Metabolomics
- Gastroenterology
Background:
- Sepsis can cause acute gastrointestinal injury.
- Antibiotic use impacts intestinal microbiota and metabolites.
Purpose of the Study:
- Investigate changes in intestinal microbiota and metabolites in sepsis mice with acute gastrointestinal injury before and after antibiotic treatment.
- Explore the effects of these changes on the body.
Main Methods:
- Utilized 16S rRNA sequencing and untargeted metabolomics on mouse fecal samples.
- Assessed intestinal motility in sepsis mice after 3-day treatment.
- Employed a three-group experimental design with SPF-grade C57BL/6J male mice.
Main Results:
- Antibiotic administration altered intestinal microbiota composition and metabolite profiles.
- Significant differences in bacterial species, including Desulfovibrio, were observed.
- Intestinal microbiota composition correlated with changes in intestinal motility; benzoic acid and 4-hydroxybenzoic acid were associated with Desulfovibrio.
Conclusions:
- Antibiotic-induced changes in gut microbiota and metabolites may influence acute gastrointestinal injury severity in sepsis.
- Inhibiting Desulfovibrio and utilizing benzoic acid/4-hydroxybenzoic acid as biomarkers could mitigate inflammation in acute gastrointestinal injury.
More Related Videos
08:33Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment
Published on: November 17, 2018
08:34Investigating the Alleviating Effects of Bacillus cereus Administration on Colitis through Gut Microbiota Modulation
Published on: July 27, 2022
Related Concept Videos
01:18Dysbiosis of the Gut Microbiota
01:21Microbiota Modulation by Antibiotics