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Published on: November 2, 2018
Functional Analysis of the Transcriptional Regulator IκB-ζ in Intestinal Homeostasis
Tomoki Sasaki1, Hiroyuki Nagashima2, Atsushi Okuma1
1Laboratory of Cell Recognition and Response, Graduate School of Life Sciences, Tohoku University, Sendai, Japan.
Background:
The Toll-like receptor signaling pathway contributes to the regulation of intestinal homeostasis through interactions with commensal bacteria. Although the transcriptional regulator IκB-ζ can be induced by Toll-like receptor signaling, its role in intestinal homeostasis is still unclear.
Aims:
To investigate the role of IκB-ζ in gut homeostasis.
Methods:
DSS-administration induced colitis in control and IκB-ζ-deficient mice. The level of immunoglobulins in feces was detected by ELISA. The immunological population in lamina propria (LP) was analyzed by FACS.
Results:
IκB-ζ-deficient mice showed severe inflammatory diseases with DSS administration in the gut. The level of IgM in the feces after DSS administration was less in IκB-ζ-deficient mice compared to control mice. Upon administration of DSS, IκB-ζ-deficient mice showed exaggerated intestinal inflammation (more IFN-g-producing CD4+ T cells in LP), and antibiotic treatment canceled this inflammatory phenotype.
Conclusion:
IκB-ζ plays a crucial role in maintaining homeostasis in the gut.
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