Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bone Remodeling01:40

Bone Remodeling

39.3K
Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
39.3K
Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

5.5K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
5.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical Efficacy of Narrow-Diameter Tissue-Level Implants for Molar Replacement: A 1-Year Randomised Controlled Trial.

Journal of clinical periodontology·2026
Same author

Horizontal Ridge Augmentation Using an rhBMP-2 Loaded Volume-Stable Collagen Matrix Compared to Guided Bone Regeneration: A Preclinical In Vivo Study.

Journal of clinical periodontology·2026
Same author

Beyond plagiarism: emerging challenges in research integrity and editorial responsibility.

Journal of periodontal & implant science·2026
Same author

Lignin-Enhanced Biofoam for Gravity-Resistant Detoxification in Urban Environments.

ACS nano·2026
Same author

Case Report: An anomalous drainage of the left hepatic vein, a persistent left cranial vena cava, and an abdominal arteriovenous fistula in a Devon Rex cat.

Frontiers in veterinary science·2026
Same author

Glutathione-Responsive Inhalable Nanotherapeutics for Targeted Macrophage Reprogramming in Chronic Obstructive Pulmonary Disease.

Biomacromolecules·2026

Related Experiment Video

Updated: Nov 10, 2025

Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry
11:20

Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry

Published on: March 29, 2018

7.8K

Programmed BMP-2 release from biphasic calcium phosphates for optimal bone regeneration.

Seora Han1, Kyeong-Won Paeng2, Sohyeon Park1

  • 1Department of Chemical and Biomolecular Engineering, Yonsei University, Seoul, 03722, Republic of Korea.

Biomaterials
|April 5, 2021
PubMed
Summary

This study developed a multi-layered biphasic calcium phosphate (BCP) platform that controls bone morphogenetic protein-2 (BMP-2) release. This innovative platform enhances bone regeneration, even with very low BMP-2 doses, outperforming traditional methods.

Keywords:
(3-Aminopropyl)triethoxysilaneBiphasic calcium phosphatesBone morphogenetic proteinBone regenerationMultilayer film

More Related Videos

Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery
12:27

Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery

Published on: August 22, 2016

7.8K
Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach
10:23

Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach

Published on: August 26, 2013

14.3K

Related Experiment Videos

Last Updated: Nov 10, 2025

Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry
11:20

Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry

Published on: March 29, 2018

7.8K
Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery
12:27

Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery

Published on: August 22, 2016

7.8K
Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach
10:23

Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach

Published on: August 26, 2013

14.3K

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Controlled release of growth factors like bone morphogenetic protein-2 (BMP-2) is crucial for bone regeneration.
  • Conventional methods often result in initial burst release, limiting therapeutic efficacy.
  • Biphasic calcium phosphate (BCP) is a widely used biomaterial for bone defect repair.

Purpose of the Study:

  • To fabricate a multi-layered BCP platform for programmed BMP-2 release, minimizing initial burst release.
  • To enhance BMP-2 loading and sustain its release during osteogenic cell differentiation.
  • To evaluate the in vivo osteogenic inductive capacity of the platform with ultra-low BMP-2 doses.

Main Methods:

  • Fabrication of a multi-layered coating on BCP using organosilicate ((3-Aminopropyl)triethoxysilane - APTES) and a natural polymer-based layer-by-layer (LbL) film (collagen/heparin)5.
  • Modification of BCP surface with APTES for amine functionalization.
  • Loading of BMP-2 onto the coated BCP platform.
  • In vivo evaluation using a rabbit calvarium onlay graft model.

Main Results:

  • The multi-layered coating significantly reduced initial BMP-2 burst release by over 50%.
  • The coated platform demonstrated enhanced BMP-2 loading capacity compared to uncoated BCP.
  • Histomorphometric analysis revealed significantly greater new bone formation with the BCP platform loaded with ultra-low BMP-2 (0.01 mg/ml) compared to the conventional soaking method at 8 weeks.

Conclusions:

  • The developed multi-layered BCP platform effectively controls BMP-2 release, reducing initial burst release and sustaining delivery.
  • This programmed release strategy enhances osteogenic inductive capacity, enabling significant bone regeneration with extremely low BMP-2 concentrations.
  • The findings suggest a promising new approach for bone defect treatment using engineered biomaterial platforms.