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Published on: November 1, 2024
Facile fabrication of phospholipid-functionalized nanofiber-based barriers with enhanced anti-adhesion efficiency
Ying Mao1, Robert Guidoin2, Gaëtan Brochu2
1Key Laboratory of Textile Science and Technology, Ministry of Education, College of Textiles, Donghua University, 2999 North Renmin Road, Songjiang District, Shanghai, 201620, China; Department of Surgery, Université Laval and Division of Regenerative Medicine, CHU de Québec Research Centre, Quebec, QC, Canada.
Abstract:
Electrospun nanofibrous membranes (NFMs) have attracted considerable attention as a potential physical barrier for reducing postoperative adhesion. However, no anti-adhesion barrier can completely prevent adhesion formation. In this study, phospholipid-functionalized NFMs were readily fabricated by one-step electrospinning to obtain nanofiber-based barriers with enhanced wettability and anti-adhesion efficiency. The optimized phospholipid NFMs were shown to have a fiber diameter of 831 nm ± 135 nm that is drastically decreasing, high porosity of 87.6 % ± 1.1 %, and superior hydrophilicity. Moreover, the phospholipid NFMs with excellent cytocompatibility exhibited fibroblasts being significantly reduced (≈ 51 %) after incubation of 3 days compared to that of the NFMs (≈ 96 %), confirming long-lasting anti-adhesion capability against fibroblasts. Meanwhile, less cell adhesion and proliferation of Raw 264.7 macrophages on NFM-10Lec indicated its superior anti-inflammatory effects. Thus, the facile phospholipid-functionalized nanofibers provided a promising strategy for anti-adhesion applications.
