Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Applied Electric Fields Polarize Initiation and Growth of Endothelial Sprouts.

Journal of tissue engineering and regenerative medicine·2025
Same author

Anionic polymers amplify electrokinetic perfusion through extracellular matrices.

Frontiers in bioengineering and biotechnology·2022
Same author

Linear polysaccharides reduce production of inflammatory cytokines by LPS-stimulated bovine fibroblasts.

Veterinary immunology and immunopathology·2021
Same author

Genetic and Genomic Advances in Developmental Models: Applications for Nutrition Research.

Advances in nutrition (Bethesda, Md.)·2020
Same author

Construction and Composition of the Squid Pen from <i>Doryteuthis pealeii</i>.

The Biological bulletin·2019
Same author

Electromigration of cell surface macromolecules in DC electric fields during cell polarization and galvanotaxis.

Journal of theoretical biology·2019

Related Experiment Video

Updated: Nov 10, 2025

Enhanced Viability for Ex vivo 3D Hydrogel Cultures of Patient-Derived Xenografts in a Perfused Microfluidic Platform
12:12

Enhanced Viability for Ex vivo 3D Hydrogel Cultures of Patient-Derived Xenografts in a Perfused Microfluidic Platform

Published on: December 5, 2020

6.3K

Electrokinetic Perfusion Through Three-Dimensional Culture Reduces Cell Mortality.

Anyesha Sarkar1, Mark A Messerli1

  • 1Department of Biology and Microbiology, South Dakota State University, Brookings, South Dakota, USA.

Tissue Engineering. Part A
|April 6, 2021
PubMed
Summary

Electrokinetic perfusion (EKP) significantly reduces cell mortality in 3D cultures compared to pressure-driven perfusion (PDP). Both continuous and pulsed EKP effectively enhance cell survival, offering advantages for tissue engineering and transplantation.

Keywords:
cell viabilityelectrokinetic perfusionextracellular matrix

More Related Videos

Author Spotlight: EasyFlow - An Economical and Adaptable Perfusion Bioreactor for Large Blood Vessel Culture
06:44

Author Spotlight: EasyFlow - An Economical and Adaptable Perfusion Bioreactor for Large Blood Vessel Culture

Published on: July 28, 2023

3.9K
Electric Field-controlled Directed Migration of Neural Progenitor Cells in 2D and 3D Environments
11:15

Electric Field-controlled Directed Migration of Neural Progenitor Cells in 2D and 3D Environments

Published on: February 16, 2012

11.9K

Related Experiment Videos

Last Updated: Nov 10, 2025

Enhanced Viability for Ex vivo 3D Hydrogel Cultures of Patient-Derived Xenografts in a Perfused Microfluidic Platform
12:12

Enhanced Viability for Ex vivo 3D Hydrogel Cultures of Patient-Derived Xenografts in a Perfused Microfluidic Platform

Published on: December 5, 2020

6.3K
Author Spotlight: EasyFlow - An Economical and Adaptable Perfusion Bioreactor for Large Blood Vessel Culture
06:44

Author Spotlight: EasyFlow - An Economical and Adaptable Perfusion Bioreactor for Large Blood Vessel Culture

Published on: July 28, 2023

3.9K
Electric Field-controlled Directed Migration of Neural Progenitor Cells in 2D and 3D Environments
11:15

Electric Field-controlled Directed Migration of Neural Progenitor Cells in 2D and 3D Environments

Published on: February 16, 2012

11.9K

Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Bioengineering

Background:

  • Cell survival and proliferation depend on efficient mass transfer of nutrients and waste.
  • Limited mass transfer by diffusion poses challenges in tissue engineering and transplantation.
  • Artificial perfusion strategies are needed to overcome diffusion limitations.

Purpose of the Study:

  • To compare the efficacy of pressure-driven perfusion (PDP) and electrokinetic perfusion (EKP) in reducing cell mortality in 3D cultures.
  • To characterize electro-osmotic flow in Matrigel to match interstitial flow rates.
  • To evaluate the impact of continuous versus pulsed EKP on cell survival.

Main Methods:

  • Utilized three-dimensional Matrigel cultures to mimic tissue environments.
  • Generated interstitial flow using both pressure-driven perfusion (PDP) and electrokinetic perfusion (EKP).
  • Quantified cell mortality under different perfusion conditions (continuous/pulsed EKP, PDP).

Main Results:

  • Continuous EKP demonstrated more consistent reduction in cell mortality than PDP at comparable flow rates.
  • Pulsed EKP achieved cell mortality reduction comparable to continuous EKP.
  • Electrokinetic flow rates in Matrigel were characterized to match pressure-driven flow.

Conclusions:

  • Electrokinetic perfusion (EKP) offers significant advantages over pressure-driven perfusion (PDP) for enhancing cell survival in engineered tissues.
  • EKP is a promising method for promoting tissue viability prior to neovascularization and angiogenesis.
  • EKP may be crucial for applications in tissue engineering, transplantation, and regenerative medicine.