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[Determination of glycosylated hemoglobin HbA1c by liquid phase chromatography]
F Guyon1, M Potier, M C Coignet
1Laboràtoire de Biochimie-Toxicologie, Hôtel-Dieu, Paris.
Glycated hemoglobin A1c assay is performed using HPLC on cation exchange TSK SP 5 PW column. Separation is obtained in 11 minutes using graduated elution with sodium chloride and Bis-Tris buffer. HBA1c is resolved from the other hemoglobins (A1a, A1b, Ao, F, S, C). Reproducibility of method is quite good (cv less than 3%) and correlated with classic low pressure chromatographic analysis using cation exchange resin.
Glycated hemoglobin A1c assay is performed using HPLC on cation exchange TSK SP 5 PW column. Separation is obtained in 11 minutes using graduated elution with sodium chloride and Bis-Tris buffer. HBA1c is resolved from the other hemoglobins (A1a, A1b, Ao, F, S, C). Reproducibility of method is quite good (cv less than 3%) and correlated with classic low pressure chromatographic analysis using cation exchange resin.