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A genetically encoded sensor for measuring serotonin dynamics
Jinxia Wan1,2, Wanling Peng3, Xuelin Li1,2
1State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing, China.
Abstract:
Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating important processes in the brain. To directly visualize the release of 5-HT, we developed a genetically encoded G-protein-coupled receptor (GPCR)-activation-based 5-HT (GRAB5-HT) sensor with high sensitivity, high selectivity, subsecond kinetics and subcellular resolution. GRAB5-HT detects 5-HT release in multiple physiological and pathological conditions in both flies and mice and provides new insights into the dynamics and mechanisms of 5-HT signaling.
