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There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
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When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
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A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
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Atomic Fluorescence Spectroscopy01:29

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Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which...
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Fiber Probe for Simultaneous Mid-Infrared and Fluorescence Spectroscopic Analysis.

Andrey Bogomolov1, Tatiana Sakharova2, Iskander Usenov2,3

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|April 6, 2021
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Summary
This summary is machine-generated.

A novel multispectral fiber optic probe allows simultaneous analysis of samples using attenuated total reflection mid-infrared spectroscopy and fluorimetry. This technology provides complementary chemical information, particularly for biological tissue analysis.

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Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Biomedical Optics

Background:

  • Simultaneous analysis of samples often requires multiple probes, increasing complexity and cost.
  • Fiber optic probes offer versatility for in-situ and remote measurements.
  • Mid-infrared spectroscopy and fluorimetry provide complementary chemical and structural information.

Purpose of the Study:

  • To develop and evaluate a novel multispectral fiber optic probe.
  • To enable simultaneous attenuated total reflection mid-infrared spectroscopy and fluorimetry.
  • To demonstrate the probe's utility for analyzing diverse samples, including biological tissues.

Main Methods:

  • Design and optimization of a multispectral fiber optic probe using ray-tracing simulations.
  • Integration of attenuated total reflection (ATR) mid-infrared spectroscopy and fluorimetry.
  • Technical evaluation of probe performance against single-method probes.
  • Application to model samples of biological tissue.

Main Results:

  • The developed probe successfully integrates ATR mid-infrared spectroscopy and fluorimetry.
  • Ray-tracing simulations guided probe design optimization.
  • Output signal quality was comparable to dedicated single-method probes.
  • Complementary chemical information was obtained from the same measurement point.

Conclusions:

  • The multispectral fiber optic probe is a viable tool for simultaneous sample analysis.
  • The probe offers enhanced chemical information retrieval compared to single-method approaches.
  • Qualitative analysis of biological tissue is a key application for this technology.