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Fiber Probe for Simultaneous Mid-Infrared and Fluorescence Spectroscopic Analysis
Andrey Bogomolov1, Tatiana Sakharova2, Iskander Usenov2,3
1Samara State Technical University, Molodogvardeyskaya 244, 443100 Samara, Russia.
Analytical Chemistry
|April 6, 2021
Summary
A novel multispectral fiber optic probe allows simultaneous analysis of samples using attenuated total reflection mid-infrared spectroscopy and fluorimetry. This technology provides complementary chemical information, particularly for biological tissue analysis.
Area of Science:
- Analytical Chemistry
- Spectroscopy
- Biomedical Optics
Background:
- Simultaneous analysis of samples often requires multiple probes, increasing complexity and cost.
- Fiber optic probes offer versatility for in-situ and remote measurements.
- Mid-infrared spectroscopy and fluorimetry provide complementary chemical and structural information.
Purpose of the Study:
- To develop and evaluate a novel multispectral fiber optic probe.
- To enable simultaneous attenuated total reflection mid-infrared spectroscopy and fluorimetry.
- To demonstrate the probe's utility for analyzing diverse samples, including biological tissues.
Main Methods:
- Design and optimization of a multispectral fiber optic probe using ray-tracing simulations.
- Integration of attenuated total reflection (ATR) mid-infrared spectroscopy and fluorimetry.
- Technical evaluation of probe performance against single-method probes.
- Application to model samples of biological tissue.
Main Results:
- The developed probe successfully integrates ATR mid-infrared spectroscopy and fluorimetry.
- Ray-tracing simulations guided probe design optimization.
- Output signal quality was comparable to dedicated single-method probes.
- Complementary chemical information was obtained from the same measurement point.
Conclusions:
- The multispectral fiber optic probe is a viable tool for simultaneous sample analysis.
- The probe offers enhanced chemical information retrieval compared to single-method approaches.
- Qualitative analysis of biological tissue is a key application for this technology.
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